Sperm motility is crucial for ram fertility; however, differences in the proteome of sperm with high- and low- motility in rams (Ovis aries) has yet to be achieved. Thus, the aim of this study was to identify and characterize ram spermatozoa proteins with different abundances between high- and low- motility, and identify of proteomic markers for ram spermatozoa motility using tandem mass tag (TMT) protein labeling and LC-MS/MS. In this study, the abundance of 150 proteins in high-motility (HM) ram sperm was significantly different compared with low-motility (LM) sperm. Proteins involved in sperm motility, mitochondrial activity, and spermatogenesis showed higher abundance in HM ram spermatozoa; proteins involved in protein processing and spliceosome were more abundant in LM ram spermatozoa. In conclusion, Phosphatidylethanolamine binding protein 4 is a potential proteomic marker for ram sperm motility; CCTs/HSPs are hallmarks of the LM spermatozoa in rams. Our findings highlight the functional differences between HM and LM ejaculated spermatozoa and has identified candidate proteins of interest linked to sperm motility in rams.

Significance

Inadequate sperm motility is one of the most important reasons for male subfertility or infertility. In this study, we found that the abundance of 150 proteins in high-motility ram sperm was significantly different compared with low-motility sperm. Proteomic biomarkers were discovered to reflect the motility variation in ram spermatozoa; these biomarkers may assist in illuminating the molecular mechanisms underlying sperm motility. This study expands the potential direction for sperm quality screening and animal breeding.

中文翻译：

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使用串联质量标签（TMT）方法鉴定ram精子运动性的蛋白质组学标记物。

精子运动对公羊的繁殖至关重要。然而，公羊高运动和低运动的精子蛋白质组的差异（Ovis aries）尚未实现。因此，本研究的目的是鉴定和鉴定高和低运动力之间具有不同丰度的ram精子蛋白，并使用串联质量标签（TMT）蛋白质标记和LC-MS / MS鉴定ram精子运动性的蛋白质组学标记物。在这项研究中，高运动性（HM）精子中的150种蛋白质的丰度与低运动性（LM）精子相比有显着差异。精子活力，线粒体活性和精子发生中涉及的蛋白质在HM ram精子中显示出更高的丰度。LM ram精子中参与蛋白质加工和剪接体的蛋白质含量更高。总之，磷脂酰乙醇胺结合蛋白4是ram精子运动的潜在蛋白质组学标记。CCT / HSP是公羊中LM精子的标志。

意义

精子活力不足是男性不育或不育的最重要原因之一。在这项研究中，我们发现高运动力精子中的150种蛋白质与低运动力精子相比存在显着差异。发现蛋白质组学生物标志物可反映公羊精子活力的变化。这些生物标志物可能有助于阐明精子运动的分子机制。这项研究扩展了精子质量筛选和动物育种的潜在方向。