Selective recognition of CdTe QDs and strand displacement signal amplification-assisted label-free and homogeneous fluorescence assay of nucleic acid and protein.,Journal of Materials Chemistry B

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Selective recognition of CdTe QDs and strand displacement signal amplification-assisted label-free and homogeneous fluorescence assay of nucleic acid and protein.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2019-08-07 , DOI: 10.1039/c9tb00753a
Pingyue Hu ₁ , Xiu Wang , Long Wei , Rui Dai , Xin Yuan , Ke Huang , Piaopiao Chen

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Due to their simplicity of design and operation, homogeneous bioassays have been of great interest to researchers. Herein, a label-free and free separation fluorescence sensing platform was constructed for the determination of nucleic acid and prostate specific antigen (PSA) using CdTe QDs as the signal molecule. In our previous work, we surprisingly found that the CdTe QDs can selectively distinguish Ag+ and the C-Ag+-C complex, which was the basis of the sensor. On the basis of the selective cation exchange reaction (CER), combined with the signal amplification of the strand displacement reaction (SDR), this work was first applied for the sensitive analysis of DNA. There are two types of hairpin structures in this sensing system, including the recognition probe (HP) and Ag+, which formed the C-Ag+-C structure, and the hairpin structure formed by the helper DNA itself. In this work, target DNA can trigger the SDR that generates lots of HP-helper double-stranded DNA (dsDNA) and recycles the target DNA while releasing a large amount of Ag+, thus quenching the fluorescence signal of CdTe QDs to achieve the highly sensitive detection of DNA. In order to verify the versatility of this system using DNA as a bridge and aptamers as recognition probes, we extended the system to the detection of PSA. After examining its experimental performance, it was determined that this method displayed good analytical capability for DNA in the range of 10-13-10-10 M and PSA in the range of 10-13-10-10 g mL-1 with low 25 fM and 30 fg mL-1 limits of detection (LODs), respectively; high selectivity for both the target sequence and protein was shown. In addition, this platform was successfully used for the analysis of PSA in serum samples.

中文翻译：

CdTe QD的选择性识别和链位移信号放大辅助的核酸和蛋白质的无标记均质荧光测定。

由于其简单的设计和操作，均相生物测定法引起了研究人员的极大兴趣。在本文中，构建了无标记和自由分离的荧光传感平台，用于使用CdTe QD作为信号分子确定核酸和前列腺特异性抗原（PSA）。在我们以前的工作中，我们惊讶地发现CdTe量子点可以选择性地区分Ag +和C-Ag + -C络合物，这是传感器的基础。在选择性阳离子交换反应（CER）的基础上，结合链置换反应（SDR）的信号放大，这项工作首先用于DNA的灵敏分析。该传感系统中有两种发夹结构，包括识别探针（HP）和形成了C-Ag + -C结构的Ag +，以及由辅助DNA本身形成的发夹结构。在这项工作中，目标DNA可以触发SDR，该SDR产生大量的HP-helper双链DNA（dsDNA），并在释放大量Ag +的同时回收目标DNA，从而淬灭了CdTe QD的荧光信号，从而实现了高灵敏度DNA检测。为了验证使用DNA作为桥和适体作为识别探针的系统的多功能性，我们将系统扩展到PSA的检测。在检查了其实验性能后，确定该方法对10-13-10-10 M范围内的DNA和10-13-10-10 g mL-1范围内的PSA表现出良好的分析能力，而低25 fM和30 fg mL-1的检测限（LOD）；显示了对靶序列和蛋白质的高选择性。此外，

更新日期：2019-07-02

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