Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells.,Journal of Proteome Research

当前位置： X-MOL 学术 › J. Proteome Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-07-05 , DOI: 10.1021/acs.jproteome.9b00154
Peipei Li ₁ , Li Wang _{1,

2} , Li-Jun Di ₁

Affiliation

Protein–protein interactions (PPIs) are indispensable for the dynamic assembly of multiprotein complexes that are central players of nearly all of the intracellular biological processes, such as signaling pathways, metabolic pathways, formation of intracellular organelles, establishment of cytoplasmic skeletons, etc. Numerous approaches have been invented to study PPIs both in vivo and in vitro, including the protein-fragment complementation assay (PCA), which is a widely applied technology to study PPIs and biomolecular interactions. PCA is a technology based on the expression of the bait and prey proteins in fusion with two complementary reporter protein fragments, respectively, that will reassemble when in close proximity. The reporter protein can be the enzymes or fluorescent proteins. Recovery of the enzymatic activity or fluorescent signal can be the indicator of PPI between the bait and prey proteins. Significant effort has been invested in developing many derivatives of PCA, along with various applications, in order to address specific questions. Therefore, a prompt review of these applications is important. In this review, we will categorize these applications according to the scenarios that the PCAs were applied and expect to provide a reference guideline for the future selection of PCA methods in solving a specific problem.

中文翻译：

蛋白质片段互补分析在活细胞中生物分子相互作用和生化网络分析中的应用。

蛋白质-蛋白质相互作用（PPI）对于多蛋白质复合物的动态组装是必不可少的，而蛋白质复合物是几乎所有细胞内生物学过程（例如信号传导途径，代谢途径，细胞内细胞器的形成，细胞质骨架的建立）的核心参与者。已经发明了在体内和体外研究PPI的方法，包括蛋白质片段互补分析（PCA），这是一种广泛用于研究PPI和生物分子相互作用的技术。PCA是一种基于诱饵和猎物蛋白分别与两个互补报道蛋白片段融合表达的技术，当紧密接近时，它们将重新组装。报道蛋白可以是酶或荧光蛋白。酶活性或荧光信号的恢复可能是诱饵和猎物蛋白之间PPI的指标。为了解决特定的问题，已经投入了大量的精力来开发PCA的许多派生词以及各种应用程序。因此，迅速审查这些应用程序很重要。在本文中，我们将根据应用PCA的情况对这些应用程序进行分类，并期望为将来选择PCA方法解决特定问题提供参考指南。

更新日期：2019-07-05

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南