Ultrathin two-dimensional (2D) metal-organic framework (MOF) nanosheets have attracted numerous attention due to their unparalleled advantages such as unique structures, large surface areas, good stability and high catalytic efficiency. In this paper, 2D Cu(bpy)2(OTf)2 nanosheets (Cu-MOF, bpy = 4,4-bipyridine, OTf = trifluoromethanesulfonate) are first utilized to achieve the fluorescent detection for H2O2 and glucose with their intrinsic peroxidase-like activity. The fluorescent biosensor Cu-MOF nanosheets were achieved by the efficient catalysis of non-fluorescent thiamine (TH) to strong fluorescent thiochrome in the presence of H2O2. With the combination of glucose oxidase, highly selective and sensitive fluorescent detection for glucose could be established with a detection limit of 0.41 μM and two separate linear ranges of 10-100 μM and 100-1000 μM, respectively. Furthermore, the developed method has been applied to human serum for the quantitative determination of glucose as a clinical diagnosis indicator of diabetes mellitus.

中文翻译：

---

铜基二维金属有机骨架纳米片作为辣根过氧化物酶模拟物用于葡萄糖荧光传感

超薄二维（2D）金属有机骨架（MOF）纳米片因其结构独特、表面积大、稳定性好和催化效率高等无与伦比的优势而备受关注。在本文中，2D Cu(bpy)2(OTf)2 纳米片（Cu-MOF，bpy = 4,4-联吡啶，OTf = 三氟甲磺酸盐）首先被用来实现对 H2O2 和葡萄糖的荧光检测，它们具有内在的类过氧化物酶活动。荧光生物传感器 Cu-MOF 纳米片是通过在 H2O2 存在下将非荧光硫胺素 (TH) 有效催化为强荧光硫色素来实现的。结合葡萄糖氧化酶，可建立高选择性、灵敏的葡萄糖荧光检测，检测限为0。41 μM 和两个单独的线性范围分别为 10-100 μM 和 100-1000 μM。此外，所开发的方法已应用于人血清，用于定量测定葡萄糖作为糖尿病的临床诊断指标。