Preparation, structural characterization and bioactivity of 4-O-Methylglucuronoxylan from Artemisia sphaerocephala Krasch.,Carbohydrate Polymers

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Preparation, structural characterization and bioactivity of 4-O-Methylglucuronoxylan from Artemisia sphaerocephala Krasch.
Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2019-06-20 , DOI: 10.1016/j.carbpol.2019.115009
Yangni Deng ₁ , Qian Liu ₁ , Tiantian Dang ₁ , Guiping Gong ₁ , Xiaoyi Chen ₂ , Rui Tang ₂ , Jing Sun ₁ , Shuang Song ₃ , Linjuan Huang ₁ , Zhongfu Wang ₁

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We obtained four soluble acid xylan fractions AGP-III-A, AGP-III-B, AGP-III-C and AGP-III-D from the insoluble Artemisia sphaerocephala Krasch gum (ASKG) polysaccharide by weak alkali treatment combined with H2O2-Vc oxidative degradation. Activity studies showed that the degradation components could reduce the cell viability of several cancer cell lines in a concentration-dependent manner, especially 4-O-Methylglucuronoxylan AGP-III-C with specific molecular weight and branching degree significantly reduced cancer cells viability and induced HepG2 apoptosis, also caused mitochondrial membrane dysfunction upregulated ROS levels, and induced G0/G1 arrest in HepG2 cells by cell cycle assay. Further, AGP-III-C mediates apoptosis in HepG2 cells by upregulating MAPK phosphorylation. The structure of AGP-III-C was characterized by uronic acid reduction, permethylation with GC-MS, and 2D-NMR analysis. The structure of AGP-III-C had a linear (1→4)-linked β-Xylf residue backbone with one branched 4-O-Me-α-GlcAp attached to the main chain by a (1→2)-glycosidic bond at every two β-(1→4)-Xylf units.

中文翻译：

球形青蒿4-O-甲基葡糖醛酸木聚糖的制备，结构表征和生物活性。

我们通过弱碱处理与H2O2-Vc结合，从不溶性沙地蒿头糖（ASKG）多糖中获得了四个可溶性酸性木聚糖级分AGP-III-A，AGP-III-B，AGP-III-C和AGP-III-D。氧化降解。活性研究表明，降解组分可以以浓度依赖的方式降低几种癌细胞的细胞活力，特别是4-O-甲基葡糖醛酸木聚糖AGP-III-C具有特定的分子量和分支度，显着降低了癌细胞的活力并诱导了HepG2的表达。细胞凋亡还导致线粒体膜功能异常上调ROS水平，并通过细胞周期分析诱导HepG2细胞中G0 / G1阻滞。此外，AGP-III-C通过上调MAPK磷酸化介导HepG2细胞的凋亡。AGP-III-C的结构以尿酸还原为特征，GC-MS进行全甲基化，以及2D-NMR分析。AGP-III-C的结构具有线性（1→4）连接的β-Xylf残基主链，其中一个分支的4-O-Me-α-GlcAp通过（1→2）-糖苷键连接至主链每两个β-（1→4）-Xylf单位

更新日期：2019-06-21

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