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Enzymatic synthesis of vanillin catalysed by an eugenol oxidase
Applied Catalysis A: General ( IF 4.7 ) Pub Date : 2019-06-20 , DOI: 10.1016/j.apcata.2019.117117
Miquel García-Bofill , Peter W. Sutton , Marina Guillén , Gregorio Álvaro

Vanillin is one of the most important flavours produced in the world. Due to its increasing value and its demand, mainly in the food industry, several ways to obtain it at lower prices are under study. One of the routes is based on the oxidation of vanillyl alcohol by Eugenol oxidase (EUGO), which has high potential to be used at industrial scale owing to the high space time yields that can be obtained at lab scale (2.9 g L−1 h−1 of vanillin). Additionally, EUGO can be immobilised efficiently onto different supports (MANA-agarose, Epoxy-agarose and Purolite 8204F) which can be reused several times to perform the oxidation preserving good stability and improving more than 3-fold the biocatalyst yield.



中文翻译:

丁香酚氧化酶催化香兰素的酶促合成

香兰素是世界上最重要的香料之一。由于其价值的增加和需求的增加(主要是在食品工业中),正在研究以较低价格获得它的几种方法。其中一种途径是基于丁香酚氧化酶(EUGO)对香兰醇的氧化,由于在实验室规模(2.9 g L -1  h香草醛-1)。此外,EUGO可以有效地固定在不同的载体上(MANA-琼脂糖,环氧树脂-琼脂糖和Purolite 8204F),可以多次重复使用以进行氧化,从而保持良好的稳定性并提高三倍以上的生物催化剂产率。

更新日期:2019-06-20
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