光生物调节疗法的镇痛作用：一项体外和体内研究。,Journal of Biophotonics

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光生物调节疗法的镇痛作用：一项体外和体内研究。
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2019-07-09 , DOI: 10.1002/jbio.201900043
Luisa Zupin ₁ , Giulia Ottaviani ₁ , Katia Rupel ₁ , Matteo Biasotto ₁ , Serena Zacchigna _{1,

2} , Sergio Crovella _{1,

3} , Fulvio Celsi ₃

Affiliation

激光治疗也称为光生物调节（PBM），旨在减轻与不同病理相关的疼痛，并使用波长，辐照度和注量不同的方案进行治疗。其作用机制仍不清楚，可能直接影响疼痛传播，从而降低伤害感受器反应。在我们的研究中，我们检查了两种特定的激光波长800和970 nm的效果，这些波长广泛应用于临床，并且已知会发挥重要的镇痛作用。我们的研究结果表明线粒体是孤立的背根神经节（DRG）神经元中激光的主要目标，从而降低了三磷酸腺苷的含量并增加了活性氧的水平。具体来说，是800 nm激光波长引起的线粒体失调，即增加了超氧化物的产生和线粒体膜的潜力。当先用不同的激光方案照射DRG神经元，然后用天然瞬态受体电位阳离子通道亚家族V成员1（TRPV1）配体辣椒素刺激时，只有970 nm的波长在幅度和频率上都降低了钙响应。通过皮下注射辣椒素在小鼠体内获得了一致的结果。我们的发现表明，PBM的效果取决于所使用的波长，其中800 nm的光主要作用于线粒体代谢，而970 nm的光则对伤害性信号传递起作用。当先用不同的激光方案照射DRG神经元，然后用天然瞬态受体电位阳离子通道亚家族V成员1（TRPV1）配体辣椒素刺激时，只有970 nm的波长在幅度和频率上都降低了钙响应。通过皮下注射辣椒素在小鼠体内获得了一致的结果。我们的发现表明，PBM的效果取决于所用的波长，其中800 nm的光主要作用于线粒体代谢，而970 nm的光则对伤害性信号传递起作用。当先用不同的激光方案照射DRG神经元，然后用天然瞬态受体电位阳离子通道亚家族V成员1（TRPV1）配体辣椒素刺激时，只有970 nm的波长在幅度和频率上都降低了钙响应。通过皮下注射辣椒素在小鼠体内获得了一致的结果。我们的发现表明，PBM的效果取决于所用的波长，其中800 nm的光主要作用于线粒体代谢，而970 nm的光则对伤害性信号传递起作用。

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Analgesic effect of Photobiomodulation Therapy: An in vitro and in vivo study.

Laser therapy, also known as Photobiomodulation (PBM) is indicated to reduce pain associated with different pathologies and applied using protocols that vary in wavelength, irradiance and fluence. Its mechanisms of action are still unclear and possibly able to directly impact on pain transmission, reducing nociceptor response. In our study, we examined the effect of two specific laser wavelengths, 800 and 970 nm, extensively applied in the clinical context and known to exert important analgesic effects. Our results point to mitochondria as the primary target of laser light in isolated dorsal root ganglion (DRG) neurons, reducing adenosine triphosphate content and increasing reactive oxygen species levels. Specifically, the 800 nm laser wavelength induced mitochondrial dysregulation, that is, increased superoxide generation and mitochondrial membrane potential. When DRG neurons were firstly illuminated by the different laser protocols and then stimulated with the natural transient receptor potential cation channel subfamily V member 1 (TRPV1) ligand capsaicin, only the 970 nm wavelength reduced the calcium response, in both amplitude and frequency. Consistent results were obtained in vivo in mice, by subcutaneous injection of capsaicin. Our findings demonstrate that the effect of PBM depends on the wavelength used, with 800 nm light mainly acting on mitochondrial metabolism and 970 nm light on nociceptive signal transmission.

更新日期：2019-07-09

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