Direct Approach toward Label-Free DNA Detection by Surface-Enhanced Raman Spectroscopy: Discrimination of a Single-Base Mutation in 50 Base-Paired Double Helixes.,Analytical Chemistry

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Direct Approach toward Label-Free DNA Detection by Surface-Enhanced Raman Spectroscopy: Discrimination of a Single-Base Mutation in 50 Base-Paired Double Helixes.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-06-19 00:00:00 , DOI: 10.1021/acs.analchem.9b01887
Yang Li ₁ , Tianyang Gao ₁ , Guantong Xu ₁ , Xiaoxuan Xiang ₁ , Bing Zhao ₁ , Xiao Xia Han ₁ , Xinhua Guo _{1,

2}

Affiliation

Surface-enhanced Raman spectroscopy (SERS) has exhibited great potential in label-free DNA detection. Owing to the limitation in chain length, it is however still challenging for SERS as a routine method to explore the intrinsic structural information on unmodified DNA. Here, we develop a universal SERS-based approach toward quantification of A/G in single-stranded DNAs (12 up to 28 bases) by introducing a novel interfacial agent, dichloromethane. DNA hybridization is successfully probed as evidenced by the typical SERS bands attributed to hydrogen bonds in a hairpin structure. More importantly, enlarged space of “hot spots” in SERS enables discrimination of single-base mutation in double-stranded DNA with 100 bases, which as a proof-of-concept study will pave a new avenue for highly sensitive DNA detection in clinical applications.

中文翻译：

通过表面增强拉曼光谱法检测无标签DNA的直接方法：区分50个碱基配对的双螺旋中的单碱基突变。

表面增强拉曼光谱（SERS）在无标记DNA检测中显示出巨大潜力。由于链长的限制，作为常规方法，SERS仍是探索未修饰DNA固有结构信息的挑战。在这里，我们通过引入新型界面剂二氯甲烷，开发了一种基于通用SERS的方法来定量单链DNA（12至28个碱基）中的A / G。DNA杂交已成功探查，这可归因于发夹结构中氢键的典型SERS条带。更重要的是，SERS中“热点”的扩大空间可区分具有100个碱基的双链DNA中的单碱基突变，这作为概念验证研究将为临床应用中的高灵敏度DNA检测铺平道路。

更新日期：2019-06-19

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