Reprogramming somatic cells to pluripotency by Oct4, Sox2, Klf4, and Myc represent a paradigm for cell fate determination. Here, we report a combination of Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Essrb, and Sall4 (7F) that reprogram mouse embryonic fibroblasts or MEFs to chimera competent induced pluripotent stem cells (iPSCs) efficiently. RNA sequencing (RNA-seq) and ATAC-seq reveal distinct mechanisms for 7F induction of pluripotency. Dropout experiments further reveal a highly cooperative process among 7F to dynamically close and open chromatin loci that encode a network of transcription factors to mediate reprogramming. These results establish an alternative paradigm for reprogramming that may be useful for analyzing cell fate control.

通过Oct4，Sox2，Klf4和Myc将体细胞重编程为多能性代表了确定细胞命运的范例。在这里，我们报告了Jdp2，Jhdm1b，Mkk6，Glis1，Nanog，Essrb和Sall4的组合（7F）将小鼠胚胎成纤维细胞或MEF重新编程为能胜任嵌合体的诱导性多能干细胞（iPSC）。RNA测序（RNA-seq）和ATAC-seq揭示了7F诱导多能性的独特机制。辍学实验进一步揭示了7F之间高度协作的过程，可以动态地关闭和打开染色质基因座，该基因座编码转录因子网络以介导重编程。这些结果建立了用于重编程的替代范例，该范例对于分析细胞命运控制可能是有用的。