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Assessment of Enhanced Thermal Effect Due to Gold Nanoparticles during MR-Guided High-Intensity Focused Ultrasound (HIFU) Procedures Using a Mouse-Tumor Model
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2019-06-18 00:00:00 , DOI: 10.1021/acsbiomaterials.9b00368 Surendra B. Devarakonda 1 , Keith Stringer 2 , Marepalli Rao 3 , Matthew Myers 4 , Rupak Banerjee 1
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2019-06-18 00:00:00 , DOI: 10.1021/acsbiomaterials.9b00368 Surendra B. Devarakonda 1 , Keith Stringer 2 , Marepalli Rao 3 , Matthew Myers 4 , Rupak Banerjee 1
Affiliation
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An in vivo study was conducted using a mouse tumor model, to assess the utility of using gold nanoparticles (gNPs) during HIFU procedures to locally enhance heating at low powers. Tumors were grown using melanoma tumor cells (B16/F10) subcutaneously on the right flanks of mice (C57Bl/6J). Physiologically relevant concentrations (0 and 0.125%) of gNPs were directly injected into the tumors. Sonications at acoustic powers of 10 and 30 W were performed for a duration of 16 s inside a magnetic-resonance system. Temperature increases and lesion volumes were calculated and compared for procedures with and without gNPs. Histopathology study was conducted using a cleaved caspase 3 antibody and hematoxylin and eosin staining after removing the tumors from the mice. For an acoustic power of 30 W, end-of-sonication temperature increases of 25.4 ± 3.8 °C (0% gNP) and 42.2 ± 4.6 °C (0.125% gNP) were measured. Using cleaved caspase 3 antibody, it was observed that more than 1% of nuclei are affected in the case of 0.125% and 30 W but only 0.01% of nuclei are affected in the 0% case. For 30 W and a gNP concentration of 0.125%, a lesion volume of 0.33 ± 0.22 mm3 was obtained, while no lesion was observed without gNP’s.
中文翻译：
使用小鼠肿瘤模型评估MR引导的高强度聚焦超声（HIFU）手术过程中由于金纳米粒子引起的增强的热效应
使用小鼠肿瘤模型进行了一项体内研究，以评估在HIFU程序中使用金纳米颗粒（gNP）局部增强低功率加热的效用。使用黑素瘤肿瘤细胞（B16 / F10）在小鼠右腹侧（C57Bl / 6J）皮下生长肿瘤。将生理学上相关浓度（0和0.125％）的gNP直接注入肿瘤中。在磁共振系统内以10瓦和30瓦的声功率进行超声，持续16 s。计算了温度升高和病变体积，并比较了有和没有gNP的程序。从小鼠中取出肿瘤后，使用裂解的caspase 3抗体以及苏木精和曙红染色进行组织病理学研究。对于30 W的声功率，超声处理结束时的温度升高25.4±3。测量了8°C（0％gNP）和42.2±4.6°C（0.125％gNP）。使用裂解的胱天蛋白酶3抗体，观察到在0.125％和30W的情况下，超过1％的细胞核受到影响，而在0％的情况下，仅0.01％的细胞核受到影响。对于30 W和gNP浓度为0.125％的情况，病变体积为0.33±0.22 mm获得3个，而没有gNP则没有观察到病变。
更新日期：2019-06-18
中文翻译：
使用小鼠肿瘤模型评估MR引导的高强度聚焦超声（HIFU）手术过程中由于金纳米粒子引起的增强的热效应
使用小鼠肿瘤模型进行了一项体内研究，以评估在HIFU程序中使用金纳米颗粒（gNP）局部增强低功率加热的效用。使用黑素瘤肿瘤细胞（B16 / F10）在小鼠右腹侧（C57Bl / 6J）皮下生长肿瘤。将生理学上相关浓度（0和0.125％）的gNP直接注入肿瘤中。在磁共振系统内以10瓦和30瓦的声功率进行超声，持续16 s。计算了温度升高和病变体积，并比较了有和没有gNP的程序。从小鼠中取出肿瘤后，使用裂解的caspase 3抗体以及苏木精和曙红染色进行组织病理学研究。对于30 W的声功率，超声处理结束时的温度升高25.4±3。测量了8°C（0％gNP）和42.2±4.6°C（0.125％gNP）。使用裂解的胱天蛋白酶3抗体，观察到在0.125％和30W的情况下，超过1％的细胞核受到影响，而在0％的情况下，仅0.01％的细胞核受到影响。对于30 W和gNP浓度为0.125％的情况，病变体积为0.33±0.22 mm获得3个，而没有gNP则没有观察到病变。
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