Production of Natural 2-Phenylethanol from Glucose or Glycerol with Coupled Escherichia coli Strains Expressing l-Phenylalanine Biosynthesis Pathway and Artificial Biocascades,ACS Sustainable Chemistry & Engineering

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Production of Natural 2-Phenylethanol from Glucose or Glycerol with Coupled Escherichia coli Strains Expressing l-Phenylalanine Biosynthesis Pathway and Artificial Biocascades
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2019-06-13 00:00:00 , DOI: 10.1021/acssuschemeng.9b01569
Balaji Sundara Sekar ₁ , Benedict Ryan Lukito ₁ , Zhi Li ₁

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2-Phenylethanol (2-PE) is a fragrance widely used in food and cosmetics. Natural 2-PE is preferred in these applications but with high price and very limited availability. Microbial synthesis from glucose or glycerol is an attractive way to produce natural 2-PE, but suffers from low product titer. Escherichia coli NST74-Phe-Sty was engineered to coexpress the l-phenylalanine (l-Phe) biosynthesis pathway and enzyme cascade of l-Phe to 2-PE, producing only 0–0.17 g/L 2-PE from glucose at 22–37 °C due to the incompatibility of the temperature for enzyme expression and activity. Enhanced production of 2-PE (8.4–9.1 g/L) from glucose or glycerol was achieved by coupling of E. coli NST74-Phe expressing the l-Phe biosynthesis pathway for l-Phe production at the optimal temperature of 37 °C with E. coli T7-Sty expressing enzyme cascades of l-Phe to 2-PE at the optimal expression temperature of 22 °C and the optimal biotransformation temperature of 30 °C. The 2-PE titer is 4.7-fold higher than the best reported 2-PE concentration produced from glucose. The coupled strains approach could be generally applicable for enhancing microbial production of useful chemicals from sugars or glycerol.

中文翻译：

葡萄糖或甘油与表达l-苯丙氨酸生物合成途径的大肠杆菌菌株和人工生物级联反应生产天然2-苯乙醇

2-苯乙醇（2-PE）是一种广泛用于食品和化妆品的香料。天然2-PE在这些应用中是首选，但价格昂贵且可用性非常有限。由葡萄糖或甘油进行微生物合成是生产天然2-PE的一种有吸引力的方法，但其产品滴度较低。大肠杆菌NST74-Phe-Sty经过工程改造，可共同表达1-苯丙氨酸（1 -Phe ）生物合成途径和1 -Phe到2-PE的酶级联反应，在22–22时仅从葡萄糖产生0–0.17 g / L 2-PE由于温度不适合酶表达和活性，因此温度为37°C。通过耦合表达NPE74-Phe的大肠杆菌NST74-Phe ，可以提高葡萄糖或甘油中2-PE的产量（8.4-9.1 g / L）。升-Phe生物合成途径为升-Phe生产在37℃的最佳温度与大肠杆菌T7-麦粒肿表达的酶级联升在22℃时的最佳表达温度和最佳生物转化温度-Phe至2-PE 30°C。2-PE滴度比报道的由葡萄糖产生的最佳2-PE浓度高4.7倍。偶联菌株方法通常可用于增强微生物从糖或甘油中产生有用化学物质的能力。

更新日期：2019-06-13

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