Modulation and Visualization of EF-G Power Stroke During Ribosomal Translocation.,ChemBioChem

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Modulation and Visualization of EF-G Power Stroke During Ribosomal Translocation.
ChemBioChem ( IF 2.6 ) Pub Date : 2019-09-20 , DOI: 10.1002/cbic.201900276
Heng Yin ₁ , Miriam Gavriliuc ₂ , Ran Lin ₂ , Shoujun Xu ₁ , Yuhong Wang ₂

Affiliation

During ribosome translocation, the elongation factor EF-G undergoes large conformational change while maintaining its contact with the moving tRNA. We previously measured a power stroke accompanying EF-G catalysis, which was consistent with structural studies. However, the role of power stroke in translocation fidelity remains unclear. Here, we report quantitative measurements of the power strokes of structurally modified EF-Gs by using two different techniques and reveal the correlation between power stroke and translocation efficiency and fidelity. We discovered that the reduced power stroke only lowered the percentage of translocation but did not introduce translocation error. The established force -structure-function correlation for EF-G indicates that power stroke drives ribosomal translocation, but the mRNA reading frame is probably maintained by ribosome itself. Furthermore, the microscope detection method reported here can be simply implemented for other biochemical applications.

中文翻译：

核糖体易位过程中EF-G功率冲程的调节和可视化。

在核糖体易位期间，延伸因子EF-G经历了较大的构象变化，同时保持了其与移动的tRNA的接触。我们之前测量了伴随EF-G催化作用的动力冲程，这与结构研究一致。然而，中风在易位保真中的作用仍不清楚。在这里，我们报告通过使用两种不同的技术对结构修饰的EF-G的功率冲程进行定量测量，并揭示了功率冲程与移位效率和保真度之间的相关性。我们发现降低的动力冲程仅降低了换位的百分比，但没有引入换位误差。EF-G建立的力-结构-功能相关性表明，动力冲程驱动核糖体易位，但是mRNA的阅读框可能是由核糖体本身维持的。此外，此处报告的显微镜检测方法可以简单地用于其他生化应用。

更新日期：2019-09-21

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