Selected DNA Aptamers Influence Kinetics and Morphology in Calcium Phosphate Mineralization,ACS Biomaterials Science & Engineering

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Selected DNA Aptamers Influence Kinetics and Morphology in Calcium Phosphate Mineralization
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2019-06-12 00:00:00 , DOI: 10.1021/acsbiomaterials.9b00308
Jacob Shlaferman ₁ , Alexander Paige ₁ , Krista Meserve ₁ , Jason A. Miech ₁ , Aren E. Gerdon ₁

Affiliation

DNA aptamers previously selected as calcium phosphate mineralization templates were modified and analyzed to better understand structure–function relationships and to explore the mechanism of templated mineralization. Aptamers were created to strengthen or remove a prevalent G-quadruplex structure and were analyzed for structural stability, affinity to calcium phosphate, influence on homogeneous and heterogeneous calcium phosphate mineralization, and influence on mineral crystallinity and morphology. Aptamers were found to modulate mineralization kinetics in a concentration-dependent manner. Changes to the G-quadruplex structure affected affinity to hydroxyapatite (Ca₁₀(PO₄)₆(OH)₂, HAP) and had a substantial impact on the mineral crystallinity. We propose a model for aptamer-directed mineralization and anticipate the usefulness of these aptamer sequences in future biomimetic and biomedical applications.

中文翻译：

选定的DNA适体影响磷酸钙矿化的动力学和形态

修改并分析了以前被选作磷酸钙矿化模板的DNA适体，以更好地了解结构与功能的关系并探索模板化矿化的机制。建立适体以加强或去除普遍的G-四链体结构，并分析其结构稳定性，对磷酸钙的亲和力，对均相和非均相磷酸钙矿化的影响以及对矿物结晶度和形态的影响。发现适体以浓度依赖性方式调节矿化动力学。G-四链体结构的变化影响了对羟基磷灰石（Ca ₁₀（PO ₄）₆（OH）_2的亲和力（HAP），并且对矿物的结晶度有重大影响。我们提出了适体定向矿化的模型，并预期了这些适体序列在未来的仿生和生物医学应用中的有用性。

更新日期：2019-06-12

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