Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin.,Molecular Cell

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Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin.
Molecular Cell ( IF 14.5 ) Pub Date : 2019-06-11 , DOI: 10.1016/j.molcel.2019.05.001
Roberto Vazquez Nunez ₁ , Laura B Ruiz Avila ₂ , Stephan Gruber ₃

Affiliation

Multi-subunit SMC ATPases control chromosome superstructure and DNA topology, presumably by DNA translocation and loop extrusion. Chromosomal DNA is entrapped within the tripartite SMCkleisin ring. Juxtaposed SMC heads ("J heads") or engaged SMC heads ("E heads") split the SMCkleisin ring into "S" and "K" sub-compartments. Here, we map a DNA-binding interface to the S compartment of E heads SmcScpAB and show that head-DNA association is essential for efficient DNA translocation and for traversing highly transcribed genes in Bacillus subtilis. We demonstrate that in J heads, SmcScpAB chromosomal DNA resides in the K compartment but is absent from the S compartment. Our results imply that the DNA occupancy of the S compartment changes during the ATP hydrolysis cycle. We propose that DNA translocation involves DNA entry into and exit out of the S compartment, possibly by DNA transfer between compartments and DNA segment capture.

中文翻译：

原核凝缩蛋白中 SMC 臂间隙的瞬时 DNA 占据。

多亚基 SMC ATP 酶可能通过 DNA 易位和环挤出来控制染色体超结构和 DNA 拓扑。染色体 DNA 被捕获在三联 SMCkleisin 环内。并置的 SMC 头（“J 头”）或接合的 SMC 头（“E 头”）将 SMCkleisin 环分成“S”和“K”子隔室。在这里，我们将 DNA 结合界面映射到 E 头 SmcScpAB 的 S 区室，并表明头-DNA 关联对于有效的 DNA 易位和穿越枯草芽孢杆菌中高度转录的基因至关重要。我们证明，在 J 头中，SmcScpAB 染色体 DNA 驻留在 K 区室中，但不存在于 S 区室中。我们的结果表明，S 区室的 DNA 占用率在 ATP 水解循环期间发生变化。我们认为 DNA 易位涉及 DNA 进入和退出 S 区室，可能是通过区室之间的 DNA 转移和 DNA 片段捕获。

更新日期：2019-06-12

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