牛β-酪蛋白胶束作为疏水性黄酮类化合物的传递系统,Food Hydrocolloids

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牛β-酪蛋白胶束作为疏水性黄酮类化合物的传递系统
Food Hydrocolloids ( IF 11.0 ) Pub Date : 2019-11-01 , DOI: 10.1016/j.foodhyd.2019.06.005
Mo Li , Remco Fokkink , Yuanying Ni , J. Mieke Kleijn

摘要牛奶蛋白 β-酪蛋白 (β-CN) 是一种本质上非结构化的两亲蛋白，可自组装成胶束。柚皮素是葡萄柚中主要的疏水性黄烷酮，具有多种有益的生物学作用：例如，它具有抗氧化、抗癌和抗炎活性。本文表明柚皮素可以被包裹在β-CN胶束中。应用荧光光谱、分子对接建模、动态光散射 (DLS)、静态光散射 (SLS) 和等温滴定量热法 (ITC) 来表征柚皮素对所得胶束的蛋白质结合行为和特性的影响。柚皮素在 pH 7 和 pH 2 均与 β-CN 结合，促进具有明确尺寸分布的胶束的形成并稳定胶束。发现与纯β-CN胶束相比，含柚皮素的β-CN胶束具有较低的临界胶束浓度（CMC）和较大的聚集数（N agg ）。SLS 和多角度 DLS 结果表明纯 β-CN 胶束和含柚皮素的 β-CN 胶束结构之间存在相当大的差异。在柚皮素存在的情况下，形成的球形胶束具有相对松散的核心（“空心球”），而纯 β-CN 胶束较小，似乎是椭圆形的。值得注意的是，通过在胶束中摄取柚皮素，可以显着提高水溶液中柚皮素的浓度。这些发现得出的结论是，β-CN 胶束作为疏水性生物活性化合物的有效递送纳米载体是非常有前途的。

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Bovine beta-casein micelles as delivery systems for hydrophobic flavonoids

Abstract The milk protein β-casein (β-CN) is an intrinsically unstructured amphiphilic protein that self-assembles into micelles. Naringenin is the main hydrophobic flavanone in grapefruit and has several beneficial biological effects: it exhibits, for example, antioxidant, anticancer and anti-inflammatory activity. This paper shows that naringenin can be encapsulated in β-CN micelles. Fluorescence spectroscopy, molecular docking modelling, dynamic light scattering (DLS), static light scattering (SLS) and isothermal titration calorimetry (ITC) were applied to characterize the effect of naringenin on the protein association behavior and properties of the resulting micelles. Naringenin binds to β-CN at both pH 7 and pH 2, promotes the formation of micelles with a well-defined size distribution and stabilizes the micelles. It was found that naringenin-containing β-CN micelles have a lower critical micelle concentration (CMC) and a larger aggregation number ( N agg ) compared to pure β-CN micelles. SLS and multi-angle DLS results suggest considerable differences between the structures of pure β-CN micelles and naringenin-containing β-CN micelles. In the presence of naringenin spherical micelles were formed with a relatively loose core (“hollow sphere”), while the pure β-CN micelles are smaller and seem to be elliptic. Notably, by uptake of naringenin in the micelles, the concentration of naringenin in aqueous solution could be raised considerably. These findings lead to the conclusion that β-CN micelles are very promising as effective delivery nano-vehicles for hydrophobic bioactive compounds.

更新日期：2019-11-01

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