An Ovarian Protein Involved in Passive Avoidance of an Endoparasitoid To Evade Its Host Immune Response.,Journal of Proteome Research

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An Ovarian Protein Involved in Passive Avoidance of an Endoparasitoid To Evade Its Host Immune Response.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-06-19 , DOI: 10.1021/acs.jproteome.8b00824
Ziwen Teng ₁ , Huizi Wu ₁ , Xinhai Ye ₁ , Shijiao Xiong ₁ , Gang Xu ₁ , Fang Wang ₁ , Qi Fang ₁ , Gongyin Ye ₁

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Through a combination of transcriptomic and proteomic analyses, we identified 817 secreted ovarian proteins from an endoparasitoid wasp, Cotesia chilonis, of which five proteins are probably involved in passive evasion. The results of an encapsulation assay revealed that one of these passive evasion-associated proteins (Crp32B), a homologue of a 32-kDa protein (Crp32) from C. rubecula, could protect resin beads from being encapsulated by host hemocytes in a dose-dependent manner. Crp32B is transcribed in ovarian cells, nurse cells, follicular cells, and oocytes, and the protein is located throughout the ovary and on the egg surface. Moreover, Crp32B has antigenic similarity to several host components. These results indicate that C. chilonis may use molecular mimicry as a mechanism to avoid host cellular immune response.

中文翻译：

卵巢蛋白参与被动避开内寄生性虫卵以逃避其宿主免疫反应。

通过转录和蛋白质组分析的组合，我们从内寄生蜂黄蜂Cotesia chilonis中鉴定出817种分泌的卵巢蛋白，其中五种蛋白可能参与了被动逃避。封装测定的结果表明这些被动回避相关蛋白中的一个（Crp32B），从32-kDa蛋白（Crp32）的同源物C. rubecula，能够保护树脂珠从由主机血细胞被包封在一个剂量依赖方式。Crp32B在卵巢细胞，护士细胞，滤泡细胞和卵母细胞中转录，该蛋白位于整个卵巢和卵表面。此外，Crp32B与几种宿主成分具有抗原相似性。这些结果表明C. chilonis 可能使用分子模拟作为避免宿主细胞免疫反应的机制。

更新日期：2019-06-20

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