Photopolymerization-Induced Polymersome Rupture.,Langmuir

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Photopolymerization-Induced Polymersome Rupture.
Langmuir ( IF 3.7 ) Pub Date : 2019-06-04 00:00:00 , DOI: 10.1021/acs.langmuir.9b01463
Sophie C Larnaudie _{1,

2} , Ariane Peyret ₁ , Louis Beaute ₁ , Pierre Nassoy ₂ , Se Bastien Lecommandoux ₁

Affiliation

Poly(butadiene)-b-poly(ethylene oxide) (PBut_2.5-b-PEO_1.3) giant polymersomes were prepared using an emulsion-centrifugation method. The impact of a fast decrease of the osmotic pressure inside the lumen of giant PBut-b-PEO vesicles was studied by confocal microscopy. This osmotic imbalance was created by performing the photoinduced polymerization of acrylamide inside these giant polymersomes, mimicking cell-like confinement. Experimental conditions (irradiation time, relative concentration of monomer, and photoinitiator) were optimized to induce the fastest and highest osmotic pressure difference in bulk solution. When confined inside polymersomes with a low permeability membrane made of PBut-b-PEO copolymers, this hyper-osmotic shock induced a fast disruption of the membrane and polymersome burst. These findings, complementary to hypotonic shock approaches previously reported, are demonstrating the versatility and relevance of controlling and modulating osmotic pressure imbalance in self-assembled artificial cell systems and protocells.

中文翻译：

光聚合引起的聚合物破裂。

使用乳液离心法制备了聚丁二烯-b-聚环氧乙烷（PBut _2.5 - b -PEO _1.3）巨型聚合物囊泡。通过共聚焦显微镜研究了巨大的PBut- b -PEO囊泡腔内渗透压快速降低的影响。这种渗透失衡是通过在这些巨大的聚合物囊泡内部进行丙烯酰胺的光诱导聚合反应而产生的，模拟了类似细胞的限制。优化了实验条件（辐照时间，单体的相对浓度和光引发剂），以在本体溶液中引起最快和最高的渗透压差。当限制在由PBut- b制成的低渗透性膜的聚合物囊泡内部时-PEO共聚物，这种高渗透性休克引起了膜的快速破坏和聚合物小体的破裂。这些发现与先前报道的低渗休克方法相辅相成，证明了在自组装人工细胞系统和原细胞中控制和调节渗透压失衡的多功能性和相关性。

更新日期：2019-06-04

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