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The juxtamembrane domains of Arabidopsis CERK1, BAK1, and FLS2 play a conserved role in chitin-induced signaling.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2019-09-20 , DOI: 10.1111/jipb.12847 Qi Zhou 1 , Jun Liu 1 , Jingyi Wang 1 , Sufen Chen 1 , Lijuan Chen 1 , Jinfa Wang 1 , Hong-Bin Wang 1 , Bing Liu 1
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2019-09-20 , DOI: 10.1111/jipb.12847 Qi Zhou 1 , Jun Liu 1 , Jingyi Wang 1 , Sufen Chen 1 , Lijuan Chen 1 , Jinfa Wang 1 , Hong-Bin Wang 1 , Bing Liu 1
Affiliation
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Arabidopsis thaliana CERK1 is an essential receptor‐like kinase in the chitin signal transduction pathway. The juxtamembrane (JM) domain of CERK1 regulates the kinase activity of this receptor. Here we demonstrate that the JM domains of LysM‐RLKs, CERK1, and OsCERK1 play a functionally conserved role in the activation of chitin signaling in Arabidopsis. The C‐termini of the JM domains of both CERK1 and OsCERK1 are indispensable for their function. Moreover, after replacing the JM domain of CERK1 with that of the nonhomologous RLK, BAK1 (CJBa) or FLS2 (CJFl), the chimeric CERK1 receptors maintained their ability to activate chitin signaling in Arabidopsis. Interestingly, the heterologous expression of CJBa and CJFl did not induce cell death in Nicotiana benthamiana leaves. These results suggest that the JM domains of CERK1, BAK1, and FLS2 play a conserved role in chitin signaling via a mechanism not related to sequence homology.
中文翻译：
拟南芥CERK1，BAK1和FLS2的近膜结构域在几丁质诱导的信号传导中起保守作用。
拟南芥CERK1是几丁质信号转导途径中一种必不可少的受体样激酶。CERK1的近膜（JM）结构域调节该受体的激酶活性。在这里我们证明LysM‐RLKs，CERK1和OsCERK1的JM域在拟南芥中几丁质信号激活中起功能上保守的作用。CERK1和OsCERK1的JM域的C末端对于它们的功能是必不可少的。此外，在用非同源的RLK，BAK1（CJBa）或FLS2（CJF1）替换CERK1的JM结构域之后，嵌合的CERK1受体保持了它们激活拟南芥中的几丁质信号转导的能力。有趣的是，CJBa和CJF1的异源表达没有诱导细胞死亡。烟草benthamiana叶子。这些结果表明，CERK1，BAK1和FLS2的JM域通过与序列同源性无关的机制在几丁质信号传导中起着保守的作用。
更新日期：2019-09-20
中文翻译：
拟南芥CERK1，BAK1和FLS2的近膜结构域在几丁质诱导的信号传导中起保守作用。
拟南芥CERK1是几丁质信号转导途径中一种必不可少的受体样激酶。CERK1的近膜（JM）结构域调节该受体的激酶活性。在这里我们证明LysM‐RLKs，CERK1和OsCERK1的JM域在拟南芥中几丁质信号激活中起功能上保守的作用。CERK1和OsCERK1的JM域的C末端对于它们的功能是必不可少的。此外，在用非同源的RLK，BAK1（CJBa）或FLS2（CJF1）替换CERK1的JM结构域之后，嵌合的CERK1受体保持了它们激活拟南芥中的几丁质信号转导的能力。有趣的是，CJBa和CJF1的异源表达没有诱导细胞死亡。烟草benthamiana叶子。这些结果表明，CERK1，BAK1和FLS2的JM域通过与序列同源性无关的机制在几丁质信号传导中起着保守的作用。
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