Patterned PHEMA Films Synthesized by Redox Polymerization for Multicellular Spheroid Generation,Industrial & Engineering Chemistry Research

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Patterned PHEMA Films Synthesized by Redox Polymerization for Multicellular Spheroid Generation
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2019-06-13 , DOI: 10.1021/acs.iecr.9b01517
Yifan Yun ₁ , Ying Guan ₁ , Yongjun Zhang _{1,

2}

Affiliation

Patterned hydrogel films are attractive for the generation of 3D multicellular spheroids, a new in vitro cell model. Traditional methods for their synthesis, such as photolithography and micromolding, usually require a template and are difficult to fit into commercial cell culture plates. Here a simple method to synthesize patterned poly(2-hydroxyethyl methacrylate) (PHEMA) films were developed. First PHEMA films were synthesized in situ in the wells of cell culture plates by bulk redox polymerization of the monomer. Films with a corrugated surface were usually obtained. Fortunately a narrow range of initiator concentration was identified in which smooth films could be obtained. Then water was added to swell the films and wrinkling patterns were produced spontaneously. The shape and size of the patterns can be controlled by adjusting cross-link density and film thickness. Under optimized conditions, films with regular hexagonal patterns were obtained successfully. The patterned PHEMA films were further used to generate multicellular spheroids. The resulting spheroids exhibit high viability and narrow size distribution.

中文翻译：

氧化还原聚合合成的图案化PHEMA膜用于多细胞球体的产生

图案化的水凝胶膜对于3D多细胞球体（一种新的体外细胞模型）的产生具有吸引力。用于其合成的传统方法，例如光刻和微成型，通常需要模板，并且难以装入商业细胞培养板中。在这里，开发了一种简单的方法来合成图案化的聚（甲基丙烯酸2-羟乙酯）（PHEMA）膜。通过单体的本体氧化还原聚合在细胞培养板的孔中原位合成第一PHEMA膜。通常获得具有波纹表面的膜。幸运的是，在窄的引发剂浓度范围内，可以得到光滑的薄膜。然后加水使膜溶胀，并自发产生皱纹。可以通过调节交联密度和膜厚度来控制图案的形状和大小。在最佳条件下，成功获得了具有规则六边形图案的薄膜。图案化的PHEMA膜进一步用于产生多细胞球体。所得的球体显示出高的生存能力和狭窄的尺寸分布。

更新日期：2019-06-14

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