Patterning of micro- and nanoscale topologies and surface properties of polymer devices is of particular importance for a broad range of life science applications, including cell-adhesion assays and highly sensitive bioassays. The manufacturing of such devices necessitates cumbersome multiple-step fabrication procedures and results in surface properties which degrade over time. This critically hinders their wide-spread dissemination. Here, we simultaneously mold and surface energy pattern microstructures in off-stoichiometric thiol-ene by area-selective monomer self-assembly in a rapid micro-reaction injection molding cycle. We replicated arrays of 1,843,650 hydrophilic-in-hydrophobic femtolitre-wells with long-term stable surface properties and magnetically trapped beads with 75% and 87.2% efficiency in single- and multiple-seeding events, respectively. These results form the basis for ultrasensitive digital biosensors, specifically, and for the fabrication of medical devices and life science research tools, generally.

聚合物器件的微米和纳米级拓扑结构和表面特性的图案化对于广泛的生命科学应用尤其重要，包括细胞粘附测定和高度灵敏的生物测定。此类器件的制造需要繁琐的多步骤制造程序，并导致表面特性随时间退化。这严重阻碍了它们的广泛传播。在这里，我们通过区域选择性单体自组装在快速微反应注塑循环中同时在非化学计量硫醇烯中成型和表面能图案微结构。我们复制了 1,843,650 个具有长期稳定表面特性和磁性捕获珠的 1,843,650 个亲水-疏水飞升孔阵列，在单次和多次接种事件中的效率分别为 75% 和 87.2%，分别。这些结果构成了超灵敏数字生物传感器的基础，特别是医疗设备和生命科学研究工具的制造。