Role of Vertical Transmission of Shoot Endophytes in Root-Associated Microbiome Assembly and Heavy Metal Hyperaccumulation in Sedum alfredii,Environmental Science & Technology

当前位置： X-MOL 学术 › Environ. Sci. Technol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Role of Vertical Transmission of Shoot Endophytes in Root-Associated Microbiome Assembly and Heavy Metal Hyperaccumulation in Sedum alfredii
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2019-06-07 , DOI: 10.1021/acs.est.9b01093
Jipeng Luo ₁ , Qi Tao ₂ , Radek Jupa ₃ , Yuankun Liu ₁ , Keren Wu ₁ , Yuchao Song ₁ , Jinxing Li ₁ , Yue Huang ₁ , Linyun Zou ₁ , Yongchao Liang ₁ , Tingqiang Li ₁

Affiliation

The transmission mode of shoot-associated endophytes in hyperaccumulators and their roles in root microbiome assembly and heavy metal accumulation remain unclear. Using 16S rRNA gene profiling, we investigated the vertical transmission of shoot-associated endophytes in relation to growth and Cd/Zn accumulation of Sedum alfredii (Crassulaceae). Endophytes were transmitted from shoot cuttings to the rhizocompartment of new plants in both sterilized (γ-irradiated) and native soils. Vertical transmission was far more efficient in the sterile soil, and the transmitted endophytes have become a dominant component of the newly established root-associated microbiome. Based on 16S rRNA genes, the vertically transmitted taxa were identified as the families of Streptomycetaceae, Nocardioidaceae, Pseudonocardiaceae, and Rhizobiaceae. Abundances of Streptomycetaceae, Nocardioidaceae, and Pseudonocardiaceae were strongly correlated with increased shoot biomass and total Cd/Zn accumulation. Inoculation of S. alfredii with the synthetic bacterial community sharing the same phylogenetic relatedness with the vertically transmitted endophytes resulted in significant improvements in plant biomass, root morphology, and Cd/Zn accumulation. Our results demonstrate that successful vertical transmission of endophytes from shoots of S. alfredii to its rhizocompartments is possible, particularly in soils with attenuated microbiomes. Furthermore, the endophyte-derived microbiome plays an important role in metal hyperaccumulation.

中文翻译：

芽内生菌垂直传播在景天根系相关微生物组装和重金属超积累中的作用

芽相关内生菌在超积累植物中的传播模式及其在根微生物组组装和重金属积累中的作用仍不清楚。使用 16S rRNA 基因分析，我们研究了芽相关内生菌的垂直传播与景天（景天科）生长和 Cd/Zn 积累的关系). 在灭菌（γ-辐照）和天然土壤中，内生菌从枝条传播到新植物的根室。垂直传播在不育土壤中的效率要高得多，传播的内生菌已成为新建立的根相关微生物组的主要组成部分。基于16S rRNA基因，垂直传播的类群被鉴定为链霉菌科、诺卡氏菌科、假诺卡氏菌科和根瘤菌科。Streptomycetaceae、Nocardioidaceae 和 Pseudonocardiaceae 的丰度与增加的枝条生物量和总 Cd/Zn 积累密切相关。接种S. alfredii合成细菌群落与垂直传播的内生菌具有相同的系统发育相关性，导致植物生物量、根系形态和 Cd/Zn 积累显着改善。我们的结果表明，内生菌从S. alfredii的枝条到其根茎区室的成功垂直传播是可能的，特别是在微生物群落减弱的土壤中。此外，内生菌衍生的微生物组在金属超积累中起着重要作用。

更新日期：2019-06-07

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南