Bacterial colonization and biofilm formation on indwelling medical devices potentially cause serious and life-threatening infections. Technologies to prevent bacterial colonization and biofilm formation on biomedical devices are in great demand. Here, we report the preparation of coating monomers by conjugating rosin acid-derived maleopimaric acid quaternary ammonium cation (MPA-N⁺) with different chain of lengths allyloxy polyethylene glycol (APEG; either MW 1200 or 2400). The monomers of the cation plus its linker conjugates (APEG_1200/2400-MPA-N⁺) were further grafted onto the surface of silicone rubber slides (PDMS) via plasma/UV-induced surface polymerization to form antimicrobial and antifouling coatings. The dual-functional coatings exhibited excellent antimicrobial activities against gram-negative and positive bacteria, and they effectively prevented biofilm formation. The coatings also greatly reduced protein adsorption and platelet adhesion, indicating their excellent antifouling capability. In addition, the APEG_1200/2400-MPA-N⁺ cation coatings were biocompatible towards mammalian cells, as shown by in vitro cytotoxicity assays using rabbit erythrocytes and attached human aorta smooth muscle cells. In addition, the APEG₂₄₀₀-MPA-N⁺ coating exhibited significant anti-infective activity in a rodent subcutaneous infection model. We show that the APEG₂₄₀₀-MPA-N⁺ coating is an effective strategy for the prevention of infections associated with indwelling biomedical devices.

留置医疗设备上的细菌定植和生物膜形成可能导致严重且威胁生命的感染。防止细菌在生物医学装置上定植和形成生物膜的技术的需求量很大。在这里，我们报告了通过将松香酸衍生的马来海马酸季铵阳离子（MPA-N ⁺）与不同长度的烯丙氧基聚乙二醇（APEG; MW 1200或2400）偶联来制备涂料单体。阳离子单体及其连接物共轭物（_{APEG 1200/2400 -MPA} -N ⁺通过等离子体/紫外光诱导的表面聚合将二甲基丙烯酸乙酯（SMS）进一步接枝到硅橡胶载玻片（PDMS）的表面上，以形成抗菌和防污涂层。该双功能涂层对革兰氏阴性和阳性细菌表现出优异的抗菌活性，并有效防止了生物膜的形成。该涂层还大大降低了蛋白质的吸附和血小板的粘附性，表明它们具有出色的防污能力。此外，_{APEG 1200/2400 -MPA} -N ⁺阳离子涂层对哺乳动物细胞具有生物相容性，如使用兔红细胞和附着的人主动脉平滑肌细胞的体外细胞毒性试验所显示。此外，APEG ₂₄₀₀ -MPA-N ⁺在啮齿动物皮下感染模型中，该涂层表现出显着的抗感染活性。我们表明，APEG ₂₄₀₀ -MPA-N ⁺涂层是预防与留置生物医学设备相关的感染的有效策略。