Abstract Manipulation of spectral composition has been found to be an efficient way to influence the growth and quality of plant production in protected or controlled environments. The aim of this study was to compare the short- and long-term effects of early pretreatment with different color shades on the composition of phenolic compounds and photosynthetic pigments in Cichorium leaves. The analyses conducted immediately after color shade pretreatments at ˜40 μmol m−²s−1 (blue, red orange, white) indicated lower values of chlorophyll, anthocyanin and flavonoid indices, as well as of the majority of flavonoids, compared to those in the same plants subsequently grown for 4 weeks under higher white light with an intensity of ˜320 μmol m−²s−1. However, we observed significant differences in the contents of phenolics and pigments depending on previous color shade pretreatment in plants grown for 4 weeks in the same environment and in leaves grown during this period. The most significant effect was observed in plants pretreated with orange light, in which levels of isorhamnetin, its derivatives and feruloyl malate were increased, and kaempferol-3-(6”-acetyl)-glucoside levels were significantly decreased. Blue light pretreatment led to lower values of chlorogenic acid, caftaric acid and isorhamnetin-3-(6”-acetyl)-glucoside but a higher content of isorhamnetin-3-glucoside. Red light led to an increase in quercetin derivatives. Compared to plants grown under constant conditions, low light in the early growth phases led to the upregulation of feruloyl malate, caffeoyl malate and kaempferol derivatives. Nondestructive measurements enabled the identification of significant effects of light pretreatment on the chlorophyll contents, as well as the accumulation of flavonoids and anthocyanins in the epidermal leaf layer. The induction of specific metabolites influenced by the acclimation of plants to colored light environments may emphasize the environment‐induced biochemical responses associated with the remarkable plasticity of phenylpropanoid metabolism.

中文翻译：

---

不同色调下幼苗的预培养改变了菊苣生长后期叶中酚类化合物的积累

摘要 已发现，在受保护或受控的环境中，对光谱成分的操纵是影响植物生产的生长和质量的有效方法。本研究的目的是比较不同色调的早期预处理对菊苣叶酚类化合物和光合色素组成的短期和长期影响。在以约 40 μmol m-²s-1（蓝色、红色橙色、白色）进行色调预处理后立即进行的分析表明，与对照组相比，叶绿素、花青素和类黄酮指数以及大多数类黄酮的值较低。相同的植物随后在强度为约 320 μmol m-²s-1 的更高白光下生长 4 周。然而，我们观察到酚类物质和色素含量的显着差异取决于先前在相同环境中生长 4 周的植物和在此期间生长的叶子的颜色色调预处理。在用橙色光预处理的植物中观察到最显着的效果，其中异鼠李素、其衍生物和阿魏酰苹果酸的水平增加，山奈酚-3-（6”-乙酰基）-葡萄糖苷水平显着降低。蓝光预处理导致绿原酸、咖啡酸和异鼠李素-3-(6”-乙酰基)-葡萄糖苷值较低，但异鼠李素-3-葡萄糖苷含量较高。红光导致槲皮素衍生物增加。与在恒定条件下生长的植物相比，早期生长阶段的低光照导致阿魏酰苹果酸的上调，咖啡酰苹果酸和山奈酚衍生物。无损测量能够识别光预处理对叶绿素含量的显着影响，以及表皮叶层中黄酮类化合物和花青素的积累。受植物适应有色光环境影响的特定代谢物的诱导可能强调与苯丙烷代谢的显着可塑性相关的环境诱导的生化反应。