抑制生长抑制素（MSTN）及其两个受体（ACVR2A和ACVR2B）基因影响敲低鸡生长性状的沉默表达的比较分析。,Scientific Reports

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抑制生长抑制素（MSTN）及其两个受体（ACVR2A和ACVR2B）基因影响敲低鸡生长性状的沉默表达的比较分析。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-05-24 , DOI: 10.1038/s41598-019-44217-z
T K Bhattacharya ₁ , Renu Shukla ₁ , R N Chatterjee ₁ , S K Bhanja ₁

Affiliation

肌生长抑制素（MSTN）是一种生长分化因子8，通过抑制其胚胎发育阶段的发育过程中的细胞分化并减少成肌过程中肌成纤维细胞的生长，通过其受体ACVR2A（激活素受体IIA型）和ACVR2B（激活素受体IIB型）调节肌肉的发育。胚胎期。这项研究的目的是比较抑制肌生长抑制素，ACVR2A和ACVR2B基因表达对鸡生长特性的影响。设计，合成并克隆了Myostatin，ACVR2A和ACVR2B基因的shRNA，并将其克隆到DEST载体中。将重组分子转染到精子中，然后将转染的精子人工授精给母鸡以获得可育卵。收集可育的卵，在培养箱中温育并孵化成小鸡。ACVR2B基因的沉默显示，其体重明显高于转基因禽类中其他单个，双重和三次敲除基因的体重。与具有其他基因和对照组的鸟类相比，ACVR2B基因沉默的禽类的car体性状（如着装率，腿部肌肉％和胸肌％）最高。在ACVR2B沉默的家禽中发现最低的血清胆固醇和HDL含量。尽管不同的沉默组和对照组的差异计数没有显着差异，但该组的RBC总数最高。结论是，仅沉默MSTN的一种受体，尤其是ACVR2B，可能会在幼年期增加鸡的最高生长。

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Comparative analysis of silencing expression of myostatin (MSTN) and its two receptors (ACVR2A and ACVR2B) genes affecting growth traits in knock down chicken.

Myostatin (MSTN), a growth differentiation factor-8 regulates muscular development through its receptors, ACVR2A (Activin receptor type IIA) and ACVR2B (Activin receptor type IIB) by inhibiting cellular differentiation of developing somites during embryonic stage and diminishing myofibriller growth during post-embryonic period. The objective of this study was to compare the effect of knockdown of expression of myostatin, ACVR2A and ACVR2B genes on growth traits in chicken. The shRNAs for Myostatin, ACVR2A and ACVR2B genes were designed, synthesized and cloned in DEST vector. The recombinant molecules were transfected into the spermatozoa and transfected spermatozoa were inseminated artificially to the hens to obtain fertile eggs. The fertile eggs were collected, incubated in the incubator and hatched to chicks. Silencing of ACVR2B gene showed significantly higher body weight than other single, double and triple knock down of genes in transgenic birds. The carcass traits such as dressing%, leg muscle%, and breast muscle% were found with the highest magnitudes in birds with silencing of the ACVR2B gene as compared to the birds with that of other genes and control group. The lowest serum cholesterol and HDL content was found in ACVR2B silencing birds. The total RBC count was the highest in this group though the differential counts did not differ significantly among various silencing and control groups of birds. It is concluded that silencing of only one receptor of MSTN particularly, ACVR2B may augment the highest growth in chicken during juvenile stage. Our findings may be used as model for improving growth in other food animals and repairing muscular degenerative disorders in human and other animals.

更新日期：2019-05-24

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