In Vitro Digestion and Fermentation of Three Polysaccharide Fractions from Laminaria japonica and Their Impact on Lipid Metabolism-Associated Human Gut Microbiota,Journal of Agricultural and Food Chemistry

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In Vitro Digestion and Fermentation of Three Polysaccharide Fractions from Laminaria japonica and Their Impact on Lipid Metabolism-Associated Human Gut Microbiota
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-05-24 00:00:00 , DOI: 10.1021/acs.jafc.9b00970
Jie Gao _{1,

2} , Lianzhu Lin _{1,

2} , Zijie Chen _{1,

2} , Yongjian Cai _{1,

2} , Chuqiao Xiao _{1,

2} , Feibai Zhou _{1,

2} , Baoguo Sun _{3,

3} , Mouming Zhao _{1,

2,

3,

3}

Affiliation

Our previous study has proved that the three polysaccharide fractions from L. japonica (LP-A4, LP-A6, and LP-A8) had significantly different structure characterization. Herein, we conducted in vitro simulated digestion and fermentation to study the digestive mechanism of LP-As. The results of gastrointestinal digestion indicated that LP-A6 and LP-A8 would be easier to trap the enzyme molecules for their denser interconnected macromolecule network compared with LP-A4. Fermentation of LP-As by human gut microbiota, especially for LP-A8, generated a large amount of short-chain fatty acids (SCFAs), which could upregulate the abundance of Firmicutes (Lachnoclostridium and Eubacterium). The high content of sulfate and highly branched sugar residue of LP-A8 might help it be easily used by Firmicutes in gut microbiota of hyperlipidemic patients. Functional analysis revealed that the increased metabolic activities of glycerophospholipid metabolism, ether lipid metabolism, and fatty acid metabolism induced by LP-A8 treatment were closely associated with metabolic syndromes and hyperlipidemia.

中文翻译：

海带三种多糖组分的体外消化和发酵及其对脂质代谢相关的人类肠道微生物群的影响

我们之前的研究证明，来自日本百合的三种多糖组分（LP-A4、LP-A6和LP-A8）具有显着不同的结构特征。在此，我们进行了体外模拟消化和发酵来研究LP-As的消化机制。胃肠道消化结果表明，与LP-A4相比，LP-A6和LP-A8由于其大分子网络更密集，更容易捕获酶分子。人类肠道微生物群发酵 LP-A，尤其是 LP-A8，产生大量短链脂肪酸 (SCFA)，可以上调厚壁菌门（毛梭菌和真杆菌）的丰度。 LP-A8 的高硫酸含量和高度支化的糖残基可能有助于其更容易被高脂血症患者肠道微生物群中的厚壁菌门利用。功能分析显示，LP-A8治疗引起的甘油磷脂代谢、醚脂代谢和脂肪酸代谢活性增强与代谢综合征和高脂血症密切相关。

更新日期：2019-05-24

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