β-葡聚糖接枝微胶囊，一种研究微生物细胞壁多糖免疫调节作用的工具。,Bioconjugate Chemistry

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β-葡聚糖接枝微胶囊，一种研究微生物细胞壁多糖免疫调节作用的工具。
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2019-06-07 , DOI: 10.1021/acs.bioconjchem.9b00304
Kawthar Bouchemal ₁ , Sarah Sze Wah Wong ₂ , Nicolas Huang ₁ , Janet Anne Willment ₃ , Jean-Paul Latgé ₂ , Vishukumar Aimanianda ₂

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β-（1,3）-葡聚糖是细菌以及真菌细胞壁的抗原成分之一。我们设计了与β-（1,3）-葡聚糖连接的微胶囊（MCs），以研究其免疫调节作用。通过分别在有机相和水相中的二酰氯（癸二酰氯和对苯二酰氯）和二亚乙基三胺之间的界面缩聚获得MC。首先设计平面膜以优化单体组成并检查膜形成的动力学。然后通过使用优化的单体组成并在水相中添加荧光素，通过受控的乳化-缩聚反应获得具有水性荧光芯的MC。所选的MC配方嫁接了来自农杆菌属物种的直链β-（1,3）-葡聚糖Curdlan或分支的β-（1，3）从烟曲霉细胞壁分离的葡聚糖。这些β-（1,3）-葡聚糖移植的MC被人单核细胞衍生的巨噬细胞吞噬，并刺激了细胞因子的分泌。而且，阻断β-（1,3）-葡聚糖识别受体dectin-1并不能完全抑制这些β-（1,3）-葡聚糖移植的MC的吞噬作用，这表明其他受体参与了该过程。 β-（1,3）-葡聚糖的识别和摄取。总体而言，嫁接的MCs是研究微生物多糖的吞噬作用和免疫调节作用的有用工具。不能完全抑制这些β-（1,3）-葡聚糖移植的MC的吞噬作用，表明其他受体参与了β-（1,3）-葡聚糖的识别和摄取。总体而言，嫁接的MCs是研究微生物多糖的吞噬作用和免疫调节作用的有用工具。不能完全抑制这些β-（1,3）-葡聚糖移植的MC的吞噬作用，表明其他受体参与了β-（1,3）-葡聚糖的识别和摄取。总体而言，嫁接的MCs是研究微生物多糖的吞噬作用和免疫调节作用的有用工具。

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β-Glucan Grafted Microcapsule, a Tool for Studying the Immunomodulatory Effect of Microbial Cell Wall Polysaccharides.

β-(1,3)-Glucan is one of the antigenic components of the bacterial as well as fungal cell wall. We designed microcapsules (MCs) ligated with β-(1,3)-glucan, to study its immunomodulatory effect. The MCs were obtained by interfacial polycondensation between diacyl chloride (sebacoyl chloride and terephtaloyl chloride) and diethylenetriamine in organic and aqueous phases, respectively. Planar films were first designed to optimize monomer compositions and to examine the kinetics of film formation. MCs with aqueous fluorescent core were then obtained upon controlled emulsification-polycondensation reactions using optimized monomer compositions and adding fluorescein into the aqueous phase. The selected MC-formulation was grafted with Curdlan, a linear β-(1,3)-glucan from Agrobacterium species or branched β-(1,3)-glucan isolated from the cell wall of Aspergillus fumigatus. These β-(1,3)-glucan grafted MCs were phagocytosed by human monocyte-derived macrophages, and stimulated cytokine secretion. Moreover, the blocking of dectin-1, a β-(1,3)-glucan recognizing receptor, did not completely inhibit the phagocytosis of these β-(1,3)-glucan grafted MCs, suggesting the involvement of other receptors in the recognition and uptake of β-(1,3)-glucan. Overall, grafted MCs are a useful tool for the study of the mechanism of phagocytosis and immunomodulatory effect of the microbial polysaccharides.

更新日期：2019-05-24

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