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Determination of Electrochemical Interaction between 2‐(1H‐benzimidazol‐2‐yl) Phenol and DNA Sequences
Electroanalysis ( IF 2.7 ) Pub Date : 2019-05-22 , DOI: 10.1002/elan.201900199 Seda Nur Topkaya 1 , Arif E. Cetin 2
Electroanalysis ( IF 2.7 ) Pub Date : 2019-05-22 , DOI: 10.1002/elan.201900199 Seda Nur Topkaya 1 , Arif E. Cetin 2
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A benzimidazole derivate, 2‐(1H‐benzimidazol‐2‐yl) phenol (2‐Bip) and its interaction mechanism with sequence specific DNA was examined with Differential Pulse Voltammetry (DPV). We, for the first time, investigated the effect of 2‐Bip on sequence specific DNA with electrochemical methods by evaluating both guanine and 2‐Bip oxidation signal changes. In the study, probe sequences were immobilized to the surface of the electrodes and then hybridization was achieved by sending the complementary target onto the probe modified electrodes. Following the hybridization, 2‐Bip solution was interacted with probe and hybrid sequences to see the effect of 2‐Bip on different DNA sequences. The binding constant (K), toxicity (S%) and thermodynamic parameters, i. e., Gibbs free energy (ΔG°) of 2‐Bip‐DNA complexes were evaluated. K was calculated as 5×105 and the change in the ΔG° was found as −32.50 kJ mol−1, which are consistent well with the literature. Furthermore, S% showed that 2‐Bip is moderately toxic to single stranded DNA (ssDNA) and toxic to double stranded DNA (dsDNA). From our experimental data, we made four conclusions (i) 2‐Bip affects both ssDNA and dsDNA, (ii) 2‐Bip interaction mode with DNA could be non‐covalent interactions, (iii) 2‐Bip could be used as new DNA hybridization indicator due to its distinct effects on ssDNA and dsDNA, (iv) 2‐Bip could be used as a drug molecule for its DNA effect.
中文翻译：
2-（1H-苯并咪唑-2-基）苯酚与DNA序列之间的电化学相互作用的确定
用差示脉冲伏安法（DPV）检测了苯并咪唑衍生物2-（1H-苯并咪唑-2-基）苯酚（2-Bip）及其与序列特异性DNA的相互作用机理。我们首次通过评估鸟嘌呤和2-Bip氧化信号的变化，通过电化学方法研究了2-Bip对序列特异性DNA的影响。在这项研究中，将探针序列固定在电极表面，然后通过将互补靶标发送到探针修饰的电极上来实现杂交。杂交后，将2-Bip溶液与探针和杂交序列相互作用，以查看2-Bip对不同DNA序列的影响。结合常数（K），毒性（S％）和热力学参数，即。例如，评估了2-Bip-DNA复合物的吉布斯自由能（ΔG°）。K计算为5×10由图5可知，ΔG°的变化为-32.50kJ mol -1，与文献一致。此外，S％表明2-Bip对单链DNA（ssDNA）具有中等毒性，而对双链DNA（dsDNA）具有毒性。根据我们的实验数据，我们得出了四个结论（i）2-Bip同时影响ssDNA和dsDNA，（ii）2-Bip与DNA的相互作用方式可能是非共价相互作用，（iii）2-Bip可以用作新的DNA杂交指示剂由于其对ssDNA和dsDNA的独特影响，（iv）2-Bip可用作具有DNA效应的药物分子。
更新日期：2019-05-22
中文翻译：
2-（1H-苯并咪唑-2-基）苯酚与DNA序列之间的电化学相互作用的确定
用差示脉冲伏安法（DPV）检测了苯并咪唑衍生物2-（1H-苯并咪唑-2-基）苯酚（2-Bip）及其与序列特异性DNA的相互作用机理。我们首次通过评估鸟嘌呤和2-Bip氧化信号的变化，通过电化学方法研究了2-Bip对序列特异性DNA的影响。在这项研究中，将探针序列固定在电极表面，然后通过将互补靶标发送到探针修饰的电极上来实现杂交。杂交后，将2-Bip溶液与探针和杂交序列相互作用，以查看2-Bip对不同DNA序列的影响。结合常数（K），毒性（S％）和热力学参数，即。例如，评估了2-Bip-DNA复合物的吉布斯自由能（ΔG°）。K计算为5×10由图5可知，ΔG°的变化为-32.50kJ mol -1，与文献一致。此外，S％表明2-Bip对单链DNA（ssDNA）具有中等毒性，而对双链DNA（dsDNA）具有毒性。根据我们的实验数据，我们得出了四个结论（i）2-Bip同时影响ssDNA和dsDNA，（ii）2-Bip与DNA的相互作用方式可能是非共价相互作用，（iii）2-Bip可以用作新的DNA杂交指示剂由于其对ssDNA和dsDNA的独特影响，（iv）2-Bip可用作具有DNA效应的药物分子。
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