基于FRET策略结合适体/氧化石墨烯复合物的抗生素多重检测的Aptasensor。,Scientific Reports

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基于FRET策略结合适体/氧化石墨烯复合物的抗生素多重检测的Aptasensor。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-05-21 , DOI: 10.1038/s41598-019-44051-3
Hyungjun Youn ₁ , Kwanghyun Lee ₁ , Jin Her ₁ , Jinseong Jeon ₂ , Jihyun Mok ₁ , Jae-In So ₃ , Sangeon Shin ₁ , Changill Ban ₁

Affiliation

为了高度选择性，灵敏和快速地筛选特定抗生素，必须开发一个多重传感平台。在这项研究中，我们设计了一种新型的荧光传感器，通过荧光共振能量转移（FRET）策略结合DNase I辅助的循环酶信号放大（CESA）方法与适体/氧化石墨烯复合物结合使用，对抗生素进行抗菌。分别将磺胺二甲氧嘧啶，卡那霉素和氨苄青霉素的适体分别与花青3（Cy3），6-羧荧光素（FAM）和花青5（Cy5）偶联，并采用氧化石墨烯（GO）淬灭这三种的荧光Cy3，FAM和Cy5的效率分别为94.36％，93.94％和96.97％。使用CESA方法进行灵敏检测，结果为2。与未放大的方法相比，信号增加了1倍。适体传感器可快速检测溶液中的抗生素，对磺胺二甲嘧啶，卡那霉素和氨苄青霉素的检出限分别为1.997、2.664和2.337 ng / mL。此外，以相似的灵敏度有效地检测了溶解在牛奶中的抗生素。多重检测测试证明，荧光修饰的适体可以彼此分开工作。结果表明，适体传感器对每种抗生素均具有高特异性，并且能够同时进行多色传感，以便同时快速筛选多种抗生素。以相似的灵敏度有效地检测了溶解在牛奶中的抗生素。多重检测测试证明，荧光修饰的适体可以彼此分开工作。结果表明，适体传感器对每种抗生素均具有高特异性，并且能够同时进行多色传感，以便同时快速筛选多种抗生素。以相似的灵敏度有效地检测了溶解在牛奶中的抗生素。多重检测测试证明，荧光修饰的适体可以彼此分开工作。结果表明，适体传感器对每种抗生素均具有高特异性，并且能够同时进行多色传感，以便同时快速筛选多种抗生素。

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Aptasensor for multiplex detection of antibiotics based on FRET strategy combined with aptamer/graphene oxide complex.

The development of a multiplexed sensing platform is necessary for highly selective, sensitive, and rapid screening of specific antibiotics. In this study, we designed a novel multiplex aptasensor for antibiotics by fluorescence resonance energy transfer (FRET) strategy using DNase I-assisted cyclic enzymatic signal amplification (CESA) method combined with aptamer/graphene oxide complex. The aptamers specific for sulfadimethoxine, kanamycin, and ampicillin were conjugated with Cyanine 3 (Cy3), 6-Carboxyfluorescein (FAM), and Cyanine 5 (Cy5), respectively, and graphene oxide (GO) was adopted to quench the fluorescence of the three different fluorophores with the efficiencies of 94.36%, 93.94%, and 96.97% for Cy3, FAM, and Cy5, respectively. CESA method was used for sensitive detection, resulting in a 2.1-fold increased signal compared to those of unamplified method. The aptasensor rapidly detected antibiotics in solution with limit of detection of 1.997, 2.664, and 2.337 ng/mL for sulfadimethoxine, kanamycin, and ampicillin, respectively. In addition, antibiotics dissolved in milk were efficiently detected with similar sensitivities. Multiplexed detection test proved that the fluorescently modified aptamers could work separately from each other. The results indicate that the aptasensor offers high specificity for each antibiotic and enables simultaneous and multicolor sensing for rapid screening of multiple antibiotics at the same time.

更新日期：2019-05-21

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