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Novel Dimethylhydrazine-Derived Spirolactam Fluorescent Chemodosimeter for Tracing Basal Peroxynitrite in Live Cells and Zebrafish
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-05-14 00:00:00 , DOI: 10.1021/acs.jafc.9b01298 Caiyun Liu 1 , Xue Zhang 1 , Zilu Li 1 , Yanan Chen 1 , Zihan Zhuang 1 , Pan Jia 1 , Hanchuang Zhu 1 , Yamin Yu 1 , Baocun Zhu 1 , Wenlong Sheng 2
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-05-14 00:00:00 , DOI: 10.1021/acs.jafc.9b01298 Caiyun Liu 1 , Xue Zhang 1 , Zilu Li 1 , Yanan Chen 1 , Zihan Zhuang 1 , Pan Jia 1 , Hanchuang Zhu 1 , Yamin Yu 1 , Baocun Zhu 1 , Wenlong Sheng 2
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The precise cellular function of peroxynitrite (ONOO–) in biosystems remains elusive, primarily owing to being short of ultrasensitive techniques for monitoring its intracellular distribution. In this work, a novel rhodamine B cyclic 1,2-dimethylhydrazine fluorescent chemodosimeter RDMH-PN for highly specific and ultrasensitive monitoring of basal ONOO– in biosystems was rationally designed. The fluorescence titration experiments demonstrated that RDMH-PN was capable of quantitatively detecting 0–100 nM ONOO– (limit of detection = 0.68 nM). In addition, RDMH-PN has outstanding performances of ultrafast measurement, naked-eye detection, and preeminent selectivity toward ONOO– to accurately detect intracellular basal ONOO–. Finally, it has been confirmed that RDMH-PN could not only map the intracellular basal ONOO– level by inhibition tests but also trace the fluctuations of endogenous and exogenous ONOO– levels with diverse stimulations in live cells and zebrafish.
中文翻译：
新型二甲基肼衍生的螺内酰胺荧光化学光度计，用于追踪活细胞和斑马鱼中的基础过氧亚硝酸盐
过氧亚硝酸盐（ONOO –）在生物系统中的精确细胞功能仍然难以捉摸，这主要是由于缺乏用于监测其细胞内分布的超灵敏技术。在这项工作中，一种新颖的若丹明B色环状1,2-二甲基肼荧光chemodosimeter RDMH-PN为基础的ONOO高度特异性和超灵敏监控-在生物系统被合理设计。荧光滴定实验证实RDMH-PN是能够定量检测0-100纳米ONOO - （检测限= 0.68纳米）。此外，RDMH-PN具有超快测量，肉眼检测，并朝向ONOO卓越选择性出色的表现-精确地检测胞内基础ONOO -。最后，已经证实RDMH-PN不能只图内基础ONOO -通过抑制试验水平，而且微量的内源性和外源性ONOO的波动-水平与活细胞和斑马鱼不同的刺激。
更新日期：2019-05-14
中文翻译：
新型二甲基肼衍生的螺内酰胺荧光化学光度计，用于追踪活细胞和斑马鱼中的基础过氧亚硝酸盐
过氧亚硝酸盐（ONOO –）在生物系统中的精确细胞功能仍然难以捉摸，这主要是由于缺乏用于监测其细胞内分布的超灵敏技术。在这项工作中，一种新颖的若丹明B色环状1,2-二甲基肼荧光chemodosimeter RDMH-PN为基础的ONOO高度特异性和超灵敏监控-在生物系统被合理设计。荧光滴定实验证实RDMH-PN是能够定量检测0-100纳米ONOO - （检测限= 0.68纳米）。此外，RDMH-PN具有超快测量，肉眼检测，并朝向ONOO卓越选择性出色的表现-精确地检测胞内基础ONOO -。最后，已经证实RDMH-PN不能只图内基础ONOO -通过抑制试验水平，而且微量的内源性和外源性ONOO的波动-水平与活细胞和斑马鱼不同的刺激。
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