Cover Feature: Fluorescence Quenching‐based Assay for Measuring Golgi endo‐α‐Mannosidase (Chem. Asian J. 11/2019),Chemistry - An Asian Journal

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Cover Feature: Fluorescence Quenching‐based Assay for Measuring Golgi endo‐α‐Mannosidase (Chem. Asian J. 11/2019)
Chemistry - An Asian Journal ( IF 3.5 ) Pub Date : 2019-05-14 , DOI: 10.1002/asia.201900459
Kanae Sano ₁ , Taiki Kuribara ₂ , Nozomi Ishii ₁ , Ayumi Kuroiwa ₁ , Toshitada Yoshihara ₁ , Seiji Tobita ₁ , Kiichiro Totani ₂ , Ichiro Matsuo ₁

Affiliation

A novel tool for measuring Golgi endo‐α‐mannosidase: This glycosidase catalyzes an alternative deglucosylation process for N‐glycans and plays important roles in the post‐endoplasmic reticulum quality control pathway. Here, a FRET quenching‐based probe labeled with a reporter dye at the non‐reducing end and a quencher molecule at the reducing end is reported. This probe is hydrolyzed by the glycosidase, resulting in an increased fluorescence intensity. This assay method enables a simple, sensitive and quantitative detection of enzyme activity in complex systems without glucosidase inhibitor. More information can be found in the Full Paper by Ichiro Matsuo et al. on page 1965 in Issue 11, 2019 (DOI: 10.1002/asia.201900240).

中文翻译：

封面功能：基于荧光猝灭的高尔基内切α-甘露糖苷酶测定方法（Chem.Asian J.11 / 2019）

一种测量高尔基体内切α-甘露糖苷酶的新颖工具：这种糖苷酶催化N-糖的另一种去糖基化过程，并在内质网后质控途径中发挥重要作用。此处报道了一种基于FRET淬灭的探针，该探针在非还原端标记有报告基因染料，在还原端标记有淬灭剂分子。该探针被糖苷酶水解，导致荧光强度增加。这种测定方法可以在没有葡萄糖苷酶抑制剂的复杂系统中对酶活性进行简单，灵敏和定量的检测。可以在Matsuo Ichiro等人的论文全文中找到更多信息。就在第11期，1965年2019页（：10.1002 / asia.201900240 DOI）。

更新日期：2019-05-14

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