生物催化制备表面脱乙酰壳多糖纳米纤维的方法,Green Chemistry

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生物催化制备表面脱乙酰壳多糖纳米纤维的方法
Green Chemistry ( IF 9.3 ) Pub Date : 2019-05-10 , DOI: 10.1039/c9gc00857h
Wenbo Ye _{1,

2,

3,

4,

5} , Huazhong Ma _{1,

2,

3,

4,

5} , Liang Liu _{1,

2,

3,

4,

5} , Juan Yu _{1,

2,

3,

4,

5} , Jiangli Lai _{6,

7,

8,

9,

10} , Yaowei Fang _{6,

7,

8,

9,

10} , Yimin Fan _{1,

2,

3,

4,

5}

Affiliation

通过培养schindleri schindleri MCDA01不育细菌，生产分子量约为31 kDa的粗壳多糖脱乙酰酶（一种新型细菌CDA）。所产生的CDA直接用于蟹壳α-甲壳质的表面脱乙酰作用。对于酶的生产，温育72小时后，通过深层发酵获得最大的总酶活性为150.09±1.66 U mL ^-1。EDTA和Fe ³⁺，Ba 2 ⁺，Sr 2 ⁺，K ⁺，Na ⁺和Li ^+的金属离子会增加酶的活性，而Co ²⁺或Cd ^2+会抑制酶的活性。。当几丁质在30°C和pH 7.0下用12000 U CDA处理96小时时，脱乙酰化程度（DDA）从6.46％增加到30.58％。保持了原始几丁质的高结晶度，酶促脱乙酰基反应后收率大于90％，表明CDA脱乙酰基发生在结晶几丁质原纤维表面，而没有破坏晶体结构。甲壳素纳米纤维（CDA-ChNs）的宽度大多在25到45 nm之间，长度通过在pH 3-4下经CDA处理的表面脱乙酰化甲壳质（CDA-Ch）水性悬浮液超声处理而获得，长度超过几微米。细菌CDA在甲壳质上的应用为甲壳质纳米纤维的生物精制提供了一条新途径，

"点击查看英文标题和摘要"

Biocatalyzed route for the preparation of surface-deacetylated chitin nanofibers

The crude chitin deacetylase (a novel bacterial CDA) with a molecular mass of approximately 31 kDa was produced by culturing the bacteria of Acinetobacter schindleri MCDA01. The produced CDA was directly applied to surface deacetylation of crab shell α-chitin. For enzyme production, a maximum total enzyme activity of 150.09 ± 1.66 U mL⁻¹ was achieved through submerged fermentation following 72 hours of incubation. Increased enzyme activity was observed with EDTA and metal ions of Fe³⁺, Ba²⁺, Sr²⁺, K⁺, Na⁺ and Li⁺, while enzyme activity was inhibited by Co²⁺ or Cd²⁺. The degree of deacetylation (DDA) was increased from 6.46% to 30.58% when the chitin was treated by 12 000 U of CDA at 30 °C and pH 7.0 for 96 h. The high crystallinity of original chitin was maintained, with yield greater than 90% after enzymatic deacetylation, indicating that the CDA deacetylation occurred on the surface of crystalline chitin fibrils without destroying the crystal structure. The chitin nanofibers (CDA-ChNs) were obtained with widths mostly between 25 and 45 nm and lengths of more than several micrometers by sonication of the CDA-treated surface-deacetylated chitin (CDA-Ch) aqueous suspensions at pH 3–4. The application of bacterial CDA on chitin provides a new path to the biorefinery of chitin nanofibers, and the resulting CDA-ChNs might exhibit advanced performance in forming multifunctional materials because of their unique fiber size and high aspect ratio.

更新日期：2019-06-04

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