A Whole Cell E. coli Display Platform for Artificial Metalloenzymes: Poly(phenylacetylene) Production with a Rhodium–Nitrobindin Metalloprotein,ACS Catalysis

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A Whole Cell E. coli Display Platform for Artificial Metalloenzymes: Poly(phenylacetylene) Production with a Rhodium–Nitrobindin Metalloprotein
ACS Catalysis ( IF 11.3 ) Pub Date : 2018-02-21 00:00:00 , DOI: 10.1021/acscatal.7b04369
Alexander R. Grimm ₁ , Daniel F. Sauer ₂ , Tino Polen ₃ , Leilei Zhu ₁ , Takashi Hayashi ₄ , Jun Okuda ₂ , Ulrich Schwaneberg _{1,

5}

Affiliation

Whole cell catalysis is, in many cases, a prerequisite for the cost-effective production of chemicals by biotechnological means. Synthetic metal catalysts for bioorthogonal reactions can be inactivated within cells due to abundant thiol derivatives. Here, a cell surface display-based whole cell biohybrid catalyst system (termed ArMt bugs) is reported as a generally applicable platform to unify cost-effective whole cell catalysis with biohybrid catalysis. An inactivated esterase autotransporter is employed to display the nitrobindin protein scaffold with a Rh catalyst on the E. coli surface. Stereoselective polymerization of phenylacetylene yielded a high turnover number (TON) (39 × 10⁶ cell^–1) for the ArMt bugs conversion platform.

中文翻译：

用于人工金属酶的全细胞大肠杆菌展示平台：铑-硝基宾丁金属蛋白的聚（苯乙炔）生产

在许多情况下，全细胞催化是通过生物技术手段经济有效地生产化学药品的先决条件。由于丰富的硫醇衍生物，用于生物正交反应的合成金属催化剂可在细胞内失活。在此，报道了基于细胞表面展示的全细胞生物杂交催化剂系统（称为ArMt bug）作为普遍适用的平台，以统一具有成本效益的全细胞催化与生物杂交催化。使用灭活的酯酶自转运蛋白在大肠杆菌表面上展示带有Rh催化剂的硝基结合蛋白支架。苯乙炔的立体选择性聚合为ArMt bug转换平台带来了高周转率（TON）（39×10 ⁶格^–1）。

更新日期：2018-02-21

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