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Biodegradation of the Neonicotinoid Insecticide Acetamiprid by Actinomycetes Streptomyces canus CGMCC 13662 and Characterization of the Novel Nitrile Hydratase Involved
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-05-08 00:00:00 , DOI: 10.1021/acs.jafc.8b06513 Ling Guo 1 , Wen-Wan Fang 1 , Lei-Lei Guo 1 , Chuan-Fei Yao 1 , Yun-Xiu Zhao 1 , Feng Ge 2 , Yi-Jun Dai 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-05-08 00:00:00 , DOI: 10.1021/acs.jafc.8b06513 Ling Guo 1 , Wen-Wan Fang 1 , Lei-Lei Guo 1 , Chuan-Fei Yao 1 , Yun-Xiu Zhao 1 , Feng Ge 2 , Yi-Jun Dai 1
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Neonicotinoid insecticide pollution in soil and water poses serious environmental risks. Microbial biodegradation is an important neonicotinoid insecticide degradation pathway in the environment. In this study, 70.0% of the acetamiprid in a 200 mg/L solution was degraded by actinomycetes Streptomyces canus CGMCC 13662 (isolated from soil) in 48 h, and the acetamiprid degradation half-life was 27.7 h. Acetamiprid was degraded to IM-1-2 ((E)-1-(1-(((6-chloropyridin-3-yl)methyl)(methyl) amino)ethylidene)urea) through hydrolysis of the cyanoimine moiety. Gene cloning and overexpression indicated that a novel nitrile hydratase with three unusual subunits (AnhD, AnhE, and AnhA) without accessory protein mediated IM-1-2 formation. The purified nitrile hydratase responsible for degrading acetamiprid had a Km of 5.85 mmol/L and a Vmax of 15.99 U/mg. A homology model suggested that AnhD-Glu56 and AnhE-His21 play important roles in the catalytic efficiency of the nitrile hydratase. S. canus CGMCC 13662 could be used to remediate environments contaminated with acetamiprid.
中文翻译：
放线菌链霉菌CGMCC 13662对新烟碱类杀虫剂Ac虫的生物降解作用及涉及的新型腈水合酶的表征
土壤和水中的新烟碱类杀虫剂污染构成严重的环境风险。微生物的生物降解是环境中重要的新烟碱类杀虫剂降解途径。在这项研究中，放线菌丝状链霉菌CGMCC 13662（从土壤中分离）在200小时内降解了200 mg / L溶液中的70.0％的乙酰胺，而乙酰胺的降解半衰期为27.7小时。扑热息痛被降解为IM-1-2（（E）-1-（1-（（（（6-氯吡啶基-3-基）甲基）（甲基）氨基）亚乙基）脲通过氰基亚胺部分的水解而形成。基因克隆和过表达表明，具有三个异常亚基（AnhD，AnhE和AnhA）的新型腈水合酶没有辅助蛋白介导的IM-1-2形成。负责降解乙酰胺的纯化腈水合酶的K m为5.85 mmol / L，V max为15.99 U / mg。同源模型表明，AnhD-Glu56和AnhE-His21在腈水合酶的催化效率中起重要作用。S. canus CGMCC 13662可用于修复受乙酰胺污染的环境。
更新日期：2019-05-08
中文翻译：
放线菌链霉菌CGMCC 13662对新烟碱类杀虫剂Ac虫的生物降解作用及涉及的新型腈水合酶的表征
土壤和水中的新烟碱类杀虫剂污染构成严重的环境风险。微生物的生物降解是环境中重要的新烟碱类杀虫剂降解途径。在这项研究中，放线菌丝状链霉菌CGMCC 13662（从土壤中分离）在200小时内降解了200 mg / L溶液中的70.0％的乙酰胺，而乙酰胺的降解半衰期为27.7小时。扑热息痛被降解为IM-1-2（（E）-1-（1-（（（（6-氯吡啶基-3-基）甲基）（甲基）氨基）亚乙基）脲通过氰基亚胺部分的水解而形成。基因克隆和过表达表明，具有三个异常亚基（AnhD，AnhE和AnhA）的新型腈水合酶没有辅助蛋白介导的IM-1-2形成。负责降解乙酰胺的纯化腈水合酶的K m为5.85 mmol / L，V max为15.99 U / mg。同源模型表明，AnhD-Glu56和AnhE-His21在腈水合酶的催化效率中起重要作用。S. canus CGMCC 13662可用于修复受乙酰胺污染的环境。
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