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Cartography of rhodopsin-like G protein-coupled receptors across vertebrate genomes.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-05-07 , DOI: 10.1038/s41598-018-33120-8 Maiju Rinne 1 , Zia-Ur-Rehman Tanoli 1, 2 , Asifullah Khan 2 , Henri Xhaard 1
Scientific Reports ( IF 3.8 ) Pub Date : 2019-05-07 , DOI: 10.1038/s41598-018-33120-8 Maiju Rinne 1 , Zia-Ur-Rehman Tanoli 1, 2 , Asifullah Khan 2 , Henri Xhaard 1
Affiliation
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We conduct a cartography of rhodopsin-like non-olfactory G protein-coupled receptors in the Ensembl database. The most recent genomic data (releases 90-92, 90 vertebrate genomes) are analyzed through the online interface and receptors mapped on phylogenetic guide trees that were constructed based on a set of ~14.000 amino acid sequences. This snapshot of genomic data suggest vertebrate genomes to harbour 142 clades of GPCRs without human orthologues. Among those, 69 have not to our knowledge been mentioned or studied previously in the literature, of which 28 are distant from existing receptors and likely new orphans. These newly identified receptors are candidates for more focused evolutionary studies such as chromosomal mapping as well for in-depth pharmacological characterization. Interestingly, we also show that 37 of the 72 human orphan (or recently deorphanized) receptors included in this study cluster into nineteen closely related groups, which implies that there are less ligands to be identified than previously anticipated. Altogether, this work has significant implications when discussing nomenclature issues for GPCRs.
中文翻译：
跨脊椎动物基因组的视紫红质样G蛋白偶联受体的制图。
我们在Ensembl数据库中对视紫红质样非嗅觉G蛋白偶联受体进行了制图。通过在线界面和映射在基于一组约14.000个氨基酸序列构建的系统发育指导树上的受体，分析了最新的基因组数据（释放90-92个，90个脊椎动物基因组）。该基因组数据快照表明，脊椎动物基因组可容纳142个GPCR进化枝，而无人类直系同源物。其中，有69个据我们所知尚未在文献中提及或研究过，其中28个与现有受体和可能的新孤儿相距甚远。这些新鉴定的受体是更集中的进化研究（如染色体作图）和深入药理学表征的候选者。有趣的是，我们还显示，这项研究中包括的72个人类孤儿（或最近去孤儿的）受体中有37个聚成19个紧密相关的组，这意味着与以前预期的相比，要鉴定的配体更少。总之，这项工作在讨论GPCR的命名问题时具有重要意义。
更新日期：2019-05-16
中文翻译：
跨脊椎动物基因组的视紫红质样G蛋白偶联受体的制图。
我们在Ensembl数据库中对视紫红质样非嗅觉G蛋白偶联受体进行了制图。通过在线界面和映射在基于一组约14.000个氨基酸序列构建的系统发育指导树上的受体，分析了最新的基因组数据（释放90-92个，90个脊椎动物基因组）。该基因组数据快照表明，脊椎动物基因组可容纳142个GPCR进化枝，而无人类直系同源物。其中，有69个据我们所知尚未在文献中提及或研究过，其中28个与现有受体和可能的新孤儿相距甚远。这些新鉴定的受体是更集中的进化研究（如染色体作图）和深入药理学表征的候选者。有趣的是，我们还显示，这项研究中包括的72个人类孤儿（或最近去孤儿的）受体中有37个聚成19个紧密相关的组，这意味着与以前预期的相比，要鉴定的配体更少。总之，这项工作在讨论GPCR的命名问题时具有重要意义。
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