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Role of ArsEFG in Roxarsone and Nitarsone Detoxification and Resistance
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2019-05-14 , DOI: 10.1021/acs.est.9b01187 Jian Chen 1 , Jun Zhang 1, 2 , Barry P. Rosen 1
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2019-05-14 , DOI: 10.1021/acs.est.9b01187 Jian Chen 1 , Jun Zhang 1, 2 , Barry P. Rosen 1
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Organoarsenical biotransformations are important components of the global cycling of arsenic. Roxarsone (3-nitro-4-hydroxybenzenearsenate or Rox(V)) and nitarsone (4-nitrobenzene arsenate or Nit(V)) are synthetic aromatic organoarsenicals used in the poultry industry as additives to prevent coccidiosis and improve feed efficiency. Here, we describe a novel pathway of resistance to roxarsone and nitarsone involving biotransformation of their trivalent forms (Rox(III)) and (Nit(III)) to the trivalent organoarsenicals HAPA(III) and pAsA(III), coupled to active extrusion of the aromatic aminobenezylarsenicals from the cells. The arsE, arsF, and arsG were cloned from the arsenic island in the chromosome of Shewanella putrefaciens 200. When expressed in Escherichia coli together, but not alone, arsEFG conferred resistance to Rox(III) and Nit(III) and decreased the accumulation of both. The cells transformed Rox(III) or Nit(III) to HAPA(III) or pAsA(III) by reducing the nitro group to an amine. Everted membrane vesicles from cells expressing arsG accumulated HAPA(III) or pAsA(III). Our data indicate that ArsE and ArsF together reduce Rox(III) or Nit(III) to HAPA(III) or pAsA(III), which are extruded from the cells by the efflux permease ArsG. Identification of the coupled pathway of ArsE, ArsF, and ArsG catalysis is a molecular description of a novel pathway for resistance to roxarsone and nitarsone.
中文翻译：
ArsEFG在罗沙松和硝苯松的排毒和抵抗中的作用
有机砷生物转化是砷全球循环的重要组成部分。Roxarsone（3-硝基-4-羟基苯砷酸盐或Rox（V））和Nitarsone（4-硝基苯砷酸盐或Nit（V））是禽类工业中用作预防球虫病和提高饲料效率的添加剂的合成芳族有机砷。在这里，我们描述了一种抗罗沙酮和尼他松的新途径，涉及其三价形式（Rox（III））和（Nit（III））到三价有机砷HAPA（III）和p AsA（III）的生物转化。从细胞中挤出芳香族氨基苯磺酸。的屁股，arsF和ARSG从砷岛的染色体克隆希瓦氏菌200。当在大肠杆菌中一起表达而不是单独表达时，arsEFG赋予了对Rox（III）和Nit（III）的抗性并减少了两者的积累。细胞通过将硝基还原为胺，将Rox（III）或Nit（III）转化为HAPA（III）或p AsA（III）。来自表达arsG的细胞的外翻膜囊泡积聚了HAPA（III）或p AsA（III）。我们的数据表明ass和ArsF共同将Rox（III）或Nit（III）还原为HAPA（III）或pAsA（III），通过外排通透酶ArsG从细胞中挤出。ArsE，ArsF和ArsG催化偶联途径的鉴定是对罗沙酮和硝苯酮耐药的新型途径的分子描述。
更新日期：2019-05-23
中文翻译：
ArsEFG在罗沙松和硝苯松的排毒和抵抗中的作用
有机砷生物转化是砷全球循环的重要组成部分。Roxarsone（3-硝基-4-羟基苯砷酸盐或Rox（V））和Nitarsone（4-硝基苯砷酸盐或Nit（V））是禽类工业中用作预防球虫病和提高饲料效率的添加剂的合成芳族有机砷。在这里，我们描述了一种抗罗沙酮和尼他松的新途径，涉及其三价形式（Rox（III））和（Nit（III））到三价有机砷HAPA（III）和p AsA（III）的生物转化。从细胞中挤出芳香族氨基苯磺酸。的屁股，arsF和ARSG从砷岛的染色体克隆希瓦氏菌200。当在大肠杆菌中一起表达而不是单独表达时，arsEFG赋予了对Rox（III）和Nit（III）的抗性并减少了两者的积累。细胞通过将硝基还原为胺，将Rox（III）或Nit（III）转化为HAPA（III）或p AsA（III）。来自表达arsG的细胞的外翻膜囊泡积聚了HAPA（III）或p AsA（III）。我们的数据表明ass和ArsF共同将Rox（III）或Nit（III）还原为HAPA（III）或pAsA（III），通过外排通透酶ArsG从细胞中挤出。ArsE，ArsF和ArsG催化偶联途径的鉴定是对罗沙酮和硝苯酮耐药的新型途径的分子描述。
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