A Highly Sensitive SPE Derivatization–UHPLC–MS Approach for Quantitative Profiling of Carotenoid-Derived Dialdehydes from Vegetables,Journal of Agricultural and Food Chemistry

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A Highly Sensitive SPE Derivatization–UHPLC–MS Approach for Quantitative Profiling of Carotenoid-Derived Dialdehydes from Vegetables
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-05-05 00:00:00 , DOI: 10.1021/acs.jafc.9b01749
Jianing Mi ₁ , Kun-Peng Jia ₁ , Aparna Balakrishna ₁ , Qitong Feng ₁ , Salim Al-Babili ₁

Affiliation

Oxidative cleavage of carotenoids leads to dialdehydes (diapocarotenoids, DIALs) in addition to the widely known apocarotenoids. DIALs are biologically active compounds that presumably impact human health and play different roles in plant development and carotenoid metabolism. However, detection of DIALs in plants is challenging due to their instability, low abundance, and poor ionization efficiency in mass spectrometry. Here, we developed a solid-phase extraction and derivatization protocol coupled with ultrahigh performance liquid chromatography–mass spectrometry for quantitative profiling of DIALs. Our method significantly enhances the sensitivity of DIAL detection with a detection limit of 0.05 pg/mg of dried food materials, allowing unambiguous profiling of 30 endogenous DIALs with C₅ to C₂₄ from vegetables. Our work provides a new and efficient approach for determining the content of DIALs from various complex matrices, paving the way for uncovering the functions of DIALs in human health and plant growth and development.

中文翻译：

用于定量分析蔬菜中类胡萝卜素衍生二醛的高灵敏度 SPE 衍生化 - UHPLC - MS 方法

除了众所周知的类胡萝卜素之外，类胡萝卜素的氧化裂解还会产生二醛（二类胡萝卜素，DIAL）。 DIAL 是具有生物活性的化合物，可能会影响人类健康，并在植物发育和类胡萝卜素代谢中发挥不同的作用。然而，由于 DIAL 不稳定、丰度低且质谱电离效率差，因此植物中 DIAL 的检测具有挑战性。在这里，我们开发了一种固相萃取和衍生化方案，与超高效液相色谱-质谱联用，用于 DIAL 的定量分析。我们的方法显着提高了 DIAL 检测的灵敏度，干燥食品材料的检测限为 0.05 pg/mg，从而可以对蔬菜中含有 C ₅至 C ₂₄的 30 种内源 DIAL 进行明确的分析。我们的工作提供了一种新的、有效的方法来确定各种复杂基质中 DIAL 的含量，为揭示 DIAL 在人类健康和植物生长发育中的功能铺平了道路。

更新日期：2019-05-05

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