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Self-Assembling Peptide-Based Multifunctional Nanofibers for Electrochemical Identification of Breast Cancer Stem-like Cells.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-04-25 00:00:00 , DOI: 10.1021/acs.analchem.8b05359 Yingying Tang 1 , Yuhao Dai 1 , Xiang Huang 2 , Lingling Li 1 , Bing Han 1 , Ya Cao 1 , Jing Zhao 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-04-25 00:00:00 , DOI: 10.1021/acs.analchem.8b05359 Yingying Tang 1 , Yuhao Dai 1 , Xiang Huang 2 , Lingling Li 1 , Bing Han 1 , Ya Cao 1 , Jing Zhao 1
Affiliation
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Cancer stem-like cells are closely related with the development and metastasis of tumors. Herein, an electrochemical method is proposed to identify stem-like cells in breast tumor. The core concept of the method is the use of multifunctional nanofibers (MNFs), which are synthesized through facile self-assembly of peptide probes. MNFs can perform three functions, specifically targeting surface biomarker to identify stem-like cells, recruiting silver nanoparticles (AgNPs) to generate electrochemical signals, and providing large amounts of reaction sites to amplify signals. Specially, breast cancer stem cells (BCSCs) are first captured by nucleolin aptamer immobilized on the electrode surface and then selectively recognized by MNFs through the binding with CD44, thereby offering a large number of azide groups for signal labeling. By tracing electrochemical signals from MNF-recruited AgNPs, the method demonstrates to detect target cells as low as 6 cells/mL within a wide linear range from 10 to 5 × 105 cells/mL. Moreover, the method can not only recognize BCSCs with high selectivity in complex environment but also monitor drug-induced stemness changes with high sensitivity, providing promising prospective clinic applications in the future.
中文翻译：
自组装基于肽的多功能纳米纤维的乳腺癌干细胞样电化学鉴定。
癌干样细胞与肿瘤的发展和转移密切相关。在本文中，提出了一种电化学方法来鉴定乳腺肿瘤中的干样细胞。该方法的核心概念是使用多功能纳米纤维（MNF），它们是通过简便的肽探针自组装合成的。MNF可以执行三种功能，特别是针对表面生物标记物以识别干细胞样细胞，募集银纳米颗粒（AgNP）以生成电化学信号以及提供大量反应位点以放大信号。特别是，乳腺癌干细胞（BCSC）首先被固定在电极表面的核仁素适体捕获，然后通过与CD44的结合被MNFs选择性识别，从而提供大量叠氮化物基团用于信号标记。5细胞/ mL。此外，该方法不仅可以在复杂环境中以高选择性识别BCSC，而且还可以高灵敏度监测药物引起的干性变化，为将来的临床应用提供了广阔的前景。
更新日期：2019-04-25
中文翻译：
自组装基于肽的多功能纳米纤维的乳腺癌干细胞样电化学鉴定。
癌干样细胞与肿瘤的发展和转移密切相关。在本文中，提出了一种电化学方法来鉴定乳腺肿瘤中的干样细胞。该方法的核心概念是使用多功能纳米纤维（MNF），它们是通过简便的肽探针自组装合成的。MNF可以执行三种功能，特别是针对表面生物标记物以识别干细胞样细胞，募集银纳米颗粒（AgNP）以生成电化学信号以及提供大量反应位点以放大信号。特别是，乳腺癌干细胞（BCSC）首先被固定在电极表面的核仁素适体捕获，然后通过与CD44的结合被MNFs选择性识别，从而提供大量叠氮化物基团用于信号标记。5细胞/ mL。此外，该方法不仅可以在复杂环境中以高选择性识别BCSC，而且还可以高灵敏度监测药物引起的干性变化，为将来的临床应用提供了广阔的前景。
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