Chemistries for Making Additive Nanolithography in OrmoComp Permissive for Cell Adhesion and Growth,ACS Applied Materials & Interfaces

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Chemistries for Making Additive Nanolithography in OrmoComp Permissive for Cell Adhesion and Growth
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2019-05-02 00:00:00 , DOI: 10.1021/acsami.9b04096
David A. Kidwell , Woo-Kyung Lee , Keith Perkins , Kathleen M. Gilpin ₁ , Thomas J. O’Shaughnessy , Jeremy T. Robinson , Paul E. Sheehan , Shawn P. Mulvaney

Affiliation

Two-photon lithography allows writing of arbitrary nanoarchitectures in photopolymers. This design flexibility opens almost limitless possibilities for biological studies, but the acrylate-based polymers frequently used do not allow for adhesion and growth of some types of cells. Indeed, we found that lithographically defined structures made from OrmoComp do not support E18 murine cortical neurons. We reacted OrmoComp structures with several diamines, thereby rendering the surfaces directly permissive for neuron attachment and growth by presenting a surface coating similar to the traditional cell biology coating achieved with poly-d-lysine (PDL) and laminin. However, in contrast to PDL–laminin coatings that cover the entire surface, the amine-terminated OrmoComp structures are orthogonally modified in deference to the surrounding glass or plastic substrate, adding yet another design element for advanced biological studies.

中文翻译：

用于在OrmoComp中制造可允许细胞粘附和生长的附加纳米光刻技术的化学方法

双光子光刻技术允许在光聚合物中写入任意纳米结构。这种设计灵活性为生物学研究提供了几乎无限的可能性，但是经常使用的丙烯酸酯类聚合物不允许某些类型的细胞粘附和生长。实际上，我们发现由OrmoComp制成的光刻定义的结构不支持E18鼠皮质神经元。我们使OrmoComp结构与几种二胺发生反应，从而通过提供类似于聚d的传统细胞生物学涂层的表面涂层，使表面直接允许神经元的附着和生长。-赖氨酸（PDL）和层粘连蛋白。但是，与覆盖整个表面的PDL-拉米宁涂层相反，端基为胺的OrmoComp结构相对于周围的玻璃或塑料基材进行了正交修饰，这为高级生物学研究增加了另一种设计元素。

更新日期：2019-05-02

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