胸腺素β-4是一种新的调节剂，用于HeLa人宫颈癌细胞中肾单位3形成初级纤毛。,Scientific Reports

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胸腺素β-4是一种新的调节剂，用于HeLa人宫颈癌细胞中肾单位3形成初级纤毛。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-05-02 , DOI: 10.1038/s41598-019-43235-1
Jae-Wook Lee ₁ , Hong Sug Kim ₂ , Eun-Yi Moon ₁

Affiliation

胸腺素β-4（Tβ4）是一种参与肌瘤的肌动蛋白调节蛋白。大多数真核细胞中都存在基于微管的初级纤毛细胞器，这可能与肿瘤细胞的转化有关。在这里，我们调查了HeLa人宫颈癌细胞中Tβ4是否会影响纤毛的发生。Tβ4的抑制减弱了初级纤毛的形成。Tβ4过表达增加了纤毛的发生频率。当以Tβ4为诱饵进行酵母双杂交试验时，我们拯救了原发性纤毛的组成成分之一肾病菌3（NPHP3）。通过GST-下拉测定法证实了Tβ4与NPHP3在哺乳动物细胞中的相互作用。通过细胞周围表面的免疫荧光染色观察到它们的细胞内共定位。另外，通过抑制NPHP3减少了纤毛细胞的数量。此外，NPHP3的表达由于抑制Tβ4而降低，但由于Tβ4的过表达而增加。两者合计，结果表明原发性纤毛的形成可以通过Tβ4与NPHP3的相互作用和/或NPHP3表达的控制来调节。这表明Tβ4是NPHP3对初级纤毛形成的新型调节剂。这也暗示肿瘤发生可能与Tβ4和/或NPHP3表达的不适当调节以正常维持原发性纤毛形成有关。这表明Tβ4是NPHP3对初级纤毛形成的新型调节剂。这也暗示肿瘤发生可能与Tβ4和/或NPHP3表达的不适当调节以正常维持原发性纤毛形成有关。这表明Tβ4是NPHP3对初级纤毛形成的新型调节剂。这也暗示肿瘤发生可能与Tβ4和/或NPHP3表达的不适当调节以正常维持原发性纤毛形成有关。

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Thymosin β-4 is a novel regulator for primary cilium formation by nephronophthisis 3 in HeLa human cervical cancer cells.

Thymosinβ-4(Tβ4) is an actin-sequestering protein involved in tumor malignancy. Primary cilia, microtubule-based organelles, are present in most eukaryotic cells, which might be related to tumor cell transformation. Here, we investigated whether ciliogenesis is affected by Tβ4 in HeLa human cervical cancer cells. The inhibition of Tβ4 attenuated primary cilia formation. The frequency of cilia was increased by Tβ4 overexpression. When yeast two-hybrid assay was performed by using Tβ4 as a bait, we rescued nephronophthisis 3(NPHP3), one of the components of primary cilia. Interaction of Tβ4 with NPHP3 in mammalian cells was confirmed by GST-pulldown assay. Their intracellular co-localization was observed by immunofluorescence staining at peripheral surface of cells. In addition, the number of ciliated cells was reduced by the inhibition of NPHP3. Moreover, NPHP3 expression was decreased by the inhibition of Tβ4 but it was increased by Tβ4 overexpression. Taken together, the results demonstrate that primary cilia formation could be regulated by Tβ4 through its interaction with NPHP3 and/or the control of NPHP3 expression. It suggests that Tβ4 is a novel regulator for primary cilia formation by NPHP3. It also suggests that tumorigenesis could be associated with inappropriate regulation of Tβ4 and/or NPHP3 expression to maintain primary cilia formation normally.

更新日期：2019-05-02

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