Fragaria vesca (F. vesca), the wild strawberry, is a diploid model for the commercial, octoploid strawberry as well as other members of the economically relevant Rosaceae family. Unlike the fruits of tomato and Arabidopsis, the fleshy fruit of strawberry is unique in that it is derived from the floral receptacle and has an external seed configuration. Thus, identification and subsequent characterization of receptacle-expressed genes may shed light on novel developmental processes or provide insight into how developmental regulation differs between receptacle-derived and ovary-derived fruits. Further, since fruit and flower tissues are the last organs to form on a plant, the development of receptacle fruit-specific promoters may provide useful molecular tools for research and application. In this work, we mined previously generated RNA-Seq datasets and identified 589 genes preferentially expressed in the strawberry receptacle versus all other profiled tissues. Promoters of a select subset of the 589 genes were isolated and their activities tested using a GUS transcriptional reporter. These promoters may now be used by the F. vesca research community for a variety of purposes, including driving expression of tissue-specific reporters, RNAi constructs, or specific genes to manipulate fruit development. Further, identified genes with receptacle-specific expression patterns, including MADS-Box and KNOX family transcription factors, are potential key regulators of fleshy fruit development and attractive candidates for functional characterization.

野草莓-Fragaria vesca（F. vesca）是商用八倍体草莓以及与经济相关的蔷薇科家族其他成员的二倍体模型。不同于番茄和拟南芥的果实，草莓的果肉果实独特，因为它是从花容器中提取的，并具有外部种子结构。因此，受体表达的基因的鉴定和随后的表征可以阐明新颖的发育过程，或者提供对源自受体的和子房的果实之间的发育调控如何不同的见解。此外，由于水果和花的组织是在植物上形成的最后器官，因此果实特有的容器的启动子的发展可能为研究和应用提供有用的分子工具。在这项工作中，我们挖掘了先前生成的RNA-Seq数据集，并确定了在草莓容器中优先表达的589个基因（相对​​于所有其他轮廓分析的组织）。分离了589个基因的选定子集的启动子，并使用GUS转录报告基因测试了它们的活性。这些启动器现在可以由F. vesca研究社区有多种用途，包括驱动组织特异性报道分子，RNAi构建体或特定基因的表达来操纵果实发育。此外，已鉴定的具有受体特异性表达模式的基因，包括MADS-Box和KNOX家族转录因子，是肉质水果发育的潜在关键调控因子，并且是功能表征的诱人候选者。