AbstractThe authors report a sensitive electrochemical aptamer-based assay for the cancer biomarker human epidermal growth factor receptor-2 (HER2). It is based on the use of MnO2 nanosheets that were functionalized with phosphate and a HER2 binding aptamer and serve as the electrochemical probe. The assay follows a sandwich protocol. A peptide that can specifically recognize HER2 was immobilized on a gold electrode for the capture of HER2. Then, the functionalized MnO2 nanosheets were linked to the electrode via the binding between HER2 and HER2 aptamer on the MnO2 nanosheets. The reaction of phosphate and aptamer on the MnO2 nanosheets with molybdate leads to the formation of redox-active molybdophosphate. This results in dual signal amplification. The generated electrochemical current was measured at 0.22 V (vs. Ag/AgCl). The assay allows HER2 to be determined in the 0.1 to 500 pg·mL−1 concentration range, and the detection limit is as low as 0.05 pg·mL−1. The assay was successfully applied for the detection of HER2 in spiked human serum samples. Graphical abstractElectrochemical detection of breast cancer biomarker human epidermal growth factor receptor-2 (HER2) is reported utilizing phosphate ions and aptamer functionalized MnO2 nanosheet as probe.

中文翻译：

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通过使用磷酸盐功能化的 MnO2 纳米片作为电化学探针，基于适体测定癌症生物标志物 HER2

摘要作者报告了一种针对癌症生物标志物人表皮生长因子受体 2 (HER2) 的基于电化学适体的灵敏检测。它基于使用磷酸和 HER2 结合适体功能化的 MnO2 纳米片作为电化学探针。该测定遵循夹心协议。一种可以特异性识别 HER2 的肽被固定在金电极上，用于捕获 HER2。然后，功能化的 MnO2 纳米片通过 HER2 和 MnO2 纳米片上的 HER2 适体之间的结合与电极连接。MnO2 纳米片上的磷酸盐和适体与钼酸盐的反应导致形成具有氧化还原活性的钼磷酸盐。这导致双信号放大。产生的电化学电流在 0.22 V（相对于 Ag/AgCl）下测量。该测定允许在 0.1 至 500 pg·mL-1 浓度范围内测定 HER2，检测限低至 0.05 pg·mL-1。该测定已成功应用于检测加标人血清样品中的 HER2。Graphical Abstract 使用磷酸根离子和适体功能化的 MnO2 纳米片作为探针，电化学检测乳腺癌生物标志物人表皮生长因子受体 2 (HER2)。