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Effect of different cocoon stifling methods on the properties of silk fibroin biomaterials.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-04-30 , DOI: 10.1038/s41598-019-43134-5 Salvador D Aznar-Cervantes 1 , Ana Pagan 1 , Beatriz Monteagudo Santesteban 1 , José L Cenis 1
Scientific Reports ( IF 3.8 ) Pub Date : 2019-04-30 , DOI: 10.1038/s41598-019-43134-5 Salvador D Aznar-Cervantes 1 , Ana Pagan 1 , Beatriz Monteagudo Santesteban 1 , José L Cenis 1
Affiliation
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Stifling treatments are applied to silk cocoons in order to kill the pupae, preventing the emergence of moths and allowing to preserve the silk during long periods of time. All of them involve the application of aggressive steps, such as sun exposure, hot steam from boiling water or hot air, during hours or even days. None of the scientific articles related to silk fibroin biomaterials has previously taken into account this fact in its section of materials and methods. In this work, the consequences of the stifling treatments most commonly used by the silk producing countries and companies are explored in depth, using fibroin films as biomaterial model. The protein degradation (visualised by SDS-PAGE) was dramatically increased in all the fibroin dissolutions produced from stifled cocoons; heavy and light chains of fibroin were specially degraded, reducing their presence along the lanes of the gel compared to the negative control (untreated fresh cocoons). Structural changes are also described for annealed silk fibroin films. The β-sheet content, analysed by means of infrared spectroscopy, was significantly higher when stifling was performed at higher temperature (70 °C and 85 °C). It is also exposed the impact of the stifling on the mechanical properties of the materials. Tensile strength and strain at break values were detected as significantly lower when this procedure was carried out by means of dry heat (85 °C) and sun exposure. On the other hand, and contrary to expectations, the proliferation of fibroblasts growing on the materials was improved by all the different stifling methods, compared to negative control, being this improvement, especially accentuated, on the films produced with fibroin purified from cocoons treated with dry heat.
中文翻译：
不同闷茧方法对丝素蛋白生物材料性能的影响
对丝茧进行窒息处理，以杀死蛹，防止蛾子的出现，并可以长期保存丝。所有这些都涉及采取激进的措施，例如阳光照射、沸水或热空气的热蒸汽，持续数小时甚至数天。之前没有一篇与丝素蛋白生物材料相关的科学文章在其材料和方法部分考虑到这一事实。在这项工作中，使用丝心蛋白膜作为生物材料模型，深入探讨了丝绸生产国和公司最常用的窒息处理的后果。闷茧产生的所有丝心蛋白溶解物中的蛋白质降解（通过 SDS-PAGE 可视化）显着增加；丝心蛋白的重链和轻链被专门降解，与阴性对照（未经处理的新鲜茧）相比，减少了它们沿着凝胶泳道的存在。还描述了退火丝素蛋白膜的结构变化。通过红外光谱分析，在较高温度（70 °C 和 85 °C）下进行熏蒸时，β-折叠含量显着较高。还暴露了窒息对材料力学性能的影响。当通过干热 (85 °C) 和阳光照射进行此过程时，检测到拉伸强度和断裂应变值显着降低。另一方面，与预期相反，与阴性对照相比，在材料上生长的成纤维细胞的增殖通过所有不同的窒息方法得到改善，这种改善在用从经处理的茧纯化的丝心蛋白产生的膜上尤其突出。干热。
更新日期：2019-04-30
中文翻译：
不同闷茧方法对丝素蛋白生物材料性能的影响
对丝茧进行窒息处理，以杀死蛹，防止蛾子的出现，并可以长期保存丝。所有这些都涉及采取激进的措施，例如阳光照射、沸水或热空气的热蒸汽，持续数小时甚至数天。之前没有一篇与丝素蛋白生物材料相关的科学文章在其材料和方法部分考虑到这一事实。在这项工作中，使用丝心蛋白膜作为生物材料模型，深入探讨了丝绸生产国和公司最常用的窒息处理的后果。闷茧产生的所有丝心蛋白溶解物中的蛋白质降解（通过 SDS-PAGE 可视化）显着增加；丝心蛋白的重链和轻链被专门降解，与阴性对照（未经处理的新鲜茧）相比，减少了它们沿着凝胶泳道的存在。还描述了退火丝素蛋白膜的结构变化。通过红外光谱分析，在较高温度（70 °C 和 85 °C）下进行熏蒸时，β-折叠含量显着较高。还暴露了窒息对材料力学性能的影响。当通过干热 (85 °C) 和阳光照射进行此过程时，检测到拉伸强度和断裂应变值显着降低。另一方面，与预期相反，与阴性对照相比，在材料上生长的成纤维细胞的增殖通过所有不同的窒息方法得到改善，这种改善在用从经处理的茧纯化的丝心蛋白产生的膜上尤其突出。干热。
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