Spectral tracing of deuterium for imaging glucose metabolism.,Nature Biomedical Engineering

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Spectral tracing of deuterium for imaging glucose metabolism.
Nature Biomedical Engineering ( IF 26.8 ) Pub Date : 2019-04-29 , DOI: 10.1038/s41551-019-0393-4
Luyuan Zhang ₁ , Lingyan Shi ₁ , Yihui Shen ₁ , Yupeng Miao ₁ , Mian Wei ₁ , Naixin Qian ₁ , Yinong Liu ₁ , Wei Min _{1,

2}

Affiliation

Cells and tissues often display pronounced spatial and dynamical metabolic heterogeneity. Common glucose-imaging techniques report glucose uptake or catabolism activity, yet do not trace the functional utilization of glucose-derived anabolic products. Here we report a microscopy technique for the optical imaging, via the spectral tracing of deuterium (STRIDE), of diverse macromolecules derived from glucose. Based on stimulated Raman-scattering imaging, STRIDE visualizes the metabolic dynamics of newly synthesized macromolecules, such as DNA, protein, lipids and glycogen, via the enrichment and distinct spectra of carbon-deuterium bonds transferred from the deuterated glucose precursor. STRIDE can also use spectral differences derived from different glucose isotopologues to visualize temporally separated glucose populations using a pulse-chase protocol. We also show that STRIDE can be used to image glucose metabolism in many mouse tissues, including tumours, brain, intestine and liver, at a detection limit of 10 mM of carbon-deuterium bonds. STRIDE provides a high-resolution and chemically informative assessment of glucose anabolic utilization.

中文翻译：

用于成像葡萄糖代谢的氘光谱示踪。

细胞和组织通常表现出明显的空间和动态代谢异质性。常见的葡萄糖成像技术报告葡萄糖摄取或分解代谢活性，但不追踪葡萄糖衍生合成代谢产物的功能利用。在这里，我们报告了一种通过氘光谱追踪 (STRIDE) 对源自葡萄糖的各种大分子进行光学成像的显微镜技术。基于受激拉曼散射成像，STRIDE 通过从氘代葡萄糖前体转移的碳-氘键的富集和不同光谱，可视化新合成的大分子（如 DNA、蛋白质、脂质和糖原）的代谢动力学。STRIDE 还可以使用源自不同葡萄糖同位素体的光谱差异，使用脉冲追踪协议可视化时间分离的葡萄糖群。我们还表明，STRIDE 可用于对许多小鼠组织（包括肿瘤、大脑、肠道和肝脏）中的葡萄糖代谢进行成像，检测限为 10 mM 的碳-氘键。STRIDE 提供了对葡萄糖合成代谢利用的高分辨率和化学信息丰富的评估。

更新日期：2019-05-16

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