XRN1相关的长非编码RNA可能有助于虫草冬虫夏草的真菌毒力和性发育。,Pest Management Science

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XRN1相关的长非编码RNA可能有助于虫草冬虫夏草的真菌毒力和性发育。
Pest Management Science ( IF 3.8 ) Pub Date : 2019-05-17 , DOI: 10.1002/ps.5453
Yulong Wang _{1,

2,

3} , Ying Shao ₁ , Yunlan Zhu ₁ , Kun Wang ₄ , Bin Ma ₄ , Qiumei Zhou ₅ , Anhui Chen ₁ , Hongwei Chen ₁

Affiliation

背景技术已经发现在哺乳动物，植物和真菌中鉴定和表征的许多长的非编码RNA（lncRNA）在生物过程中起着关键的调节作用。然而，关于lncRNA在昆虫致病性真菌中的作用知之甚少。结果通过分析昆虫病原性真菌Cord虫草中有性和无性发育的转录组，鉴定出4140个lncRNA，并在真菌发育过程中动态表达。与蛋白编码基因相比，lncRNAs的转录长度较短，外显子数量较少。可以预测各种表达的lncRNA的表达靶基因（邻近和顺式调控），这些基因在能量代谢和信号传导途径（例如“糖酵解/糖异生”和“ 为进一步研究lncRNA提供了广泛的资源和资源。©2019化学工业协会。

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XRN1-associated long non-coding RNAs may contribute to fungal virulence and sexual development in entomopathogenic fungus Cordyceps militaris.

BACKGROUND Numerous long non-coding RNAs (lncRNAs) identified and characterized in mammals, plants, and fungi have been found to play critical regulatory roles in biological processes. However, little is known about the role of lncRNAs in insect pathogenic fungi. RESULTS By profiling the transcriptomes of sexual and asexual development in the insect-pathogenic fungus Cordyceps militaris, 4140 lncRNAs were identified and found to be dynamically expressed during fungal development. The lncRNAs had shorter transcript lengths and lower numbers of exons compared to protein-coding genes. The expressed target genes (neighboring and cis-regulated) of various expressed lncRNAs were predicted, and these genes showed significant enrichment in energy metabolism and signaling pathways, such as 'Glycolysis/Gluconeogenesis' and "MAPK signaling pathway". To better understand how lncRNAs function in the fungus, xrn1, the final gene of the NMD pathway, which determines the fate of lncRNAs, was disrupted. The Δxrn1 deletion mutant displayed significant (P < 0.05) attenuation of virulence and a lower growth rate in C. militaris. Quantitative RT-PCR results revealed 10 lncRNAs with significantly higher expression, while 8 of these 10 lncRNA target genes (virulence- and sexual development-related) showed significantly lower expression in Δxrn1 compared to in the wild-type, suggesting that lncRNA expression regulates fungal virulence and sexual development by affecting gene expression. CONCLUSION These findings suggest that lncRNAs in C. militaris play important roles in the fungal infection progress and fruiting body production, providing a broad repertoire and resource for further studies of lncRNAs. © 2019 Society of Chemical Industry.

更新日期：2019-05-17

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