Enzyme Immobilization in MOF‐derived Porous NiO with Hierarchical Structure: An Efficient and Stable Enzymatic Reactor,ChemCatChem

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Enzyme Immobilization in MOF‐derived Porous NiO with Hierarchical Structure: An Efficient and Stable Enzymatic Reactor
ChemCatChem ( IF 3.8 ) Pub Date : 2019-05-20 , DOI: 10.1002/cctc.201900611
Xia Gao _{1,

2} , Yu Ding ₁ , Yude Sheng ₁ , Mancheng Hu ₁ , Quanguo Zhai ₁ , Shuni Li ₁ , Yucheng Jiang ₁ , Yu Chen ₃

Affiliation

MOF‐derived porous NiO with hierarchical structure (MHNiO) was prepared based on thermolysis of Ni metal organic framework (Ni‐MOF), which was used as carrier for the immobilization of horseradish peroxidase (HRP) and cytochrome C (Cyt c). The pore size of MHNiO was tuned as 11.8 nm to match the size of free enzyme so as to depress the aggregation of the enzymes in the pore. Meanwhile, the hierarchical structure allowed the substrates concentrated in the vicinity of the enzymes. The obtained enzymatic reactors exhibited better thermal stability, storage stability and reusability. For example, over 83 % of its original activity after 1 h incubation at 70 °C could be remained compared to 18.1 % or 38.4 % remained activity of free HRP and Cyt c, respectively; After 12 cycles of use, over 53 % of original catalytic activity of both the enzymatic reactor could be maintain. The enzymatic kinetic data (K_m, V_max, k_cat) and thermodynamic data (K_a, ΔH, ΔS and ΔG) indicated that the improved catalytic performance was attributed to the affinity, selectivity and binding of enzyme to substrate. The two enzymatic reactors could be applied in the fast degradation of 2,4‐dichlorophenol and rifaximin in artificial wastewater, a complete degradation of 2 mg ⋅ mL⁻¹ of 2,4‐dichlorophenol or 20 μg ⋅ mL⁻¹ of rifaximin was achieved in only 20 min.

中文翻译：

具有分级结构的MOF衍生多孔NiO中的酶固定化：高效稳定的酶反应器

MOF衍生的多孔NiO具有分层结构（MHNiO）是基于镍金属有机骨架（Ni-MOF）的热解制备的，用作固定辣根过氧化物酶（HRP）和细胞色素C（Cyt c）的载体。MHNiO的孔径调节为11.8 nm，以匹配游离酶的大小，从而抑制酶在孔中的聚集。同时，分层结构允许底物集中在酶附近。所获得的酶反应器表现出更好的热稳定性，储存稳定性和可重复使用性。例如，在70°C孵育1小时后，其原始活性的83％以上可以保留，而游离HRP和Cyt c的活性分别为18.1％或38.4％。使用12个周期后，两个酶反应器的原始催化活性都可以维持超过53％。酶动力学数据（K _m，V _max，k _cat）和热力学数据（K _a，ΔH，ΔS和ΔG）表明，改进的催化性能归因于酶与底物的亲和力，选择性和结合力。这两个酶反应器可用于人工废水中2,4-二氯苯酚和利福昔明的快速降解，实现了2 mg⋅mL ^-1的2,4-二氯苯酚或20μg⋅mL ^-1的利福昔明的完全降解。在仅20分钟内

更新日期：2019-05-20

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