金属-有机骨架纳米粒子辅助红细胞冷冻保存,Journal of the American Chemical Society

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金属-有机骨架纳米粒子辅助红细胞冷冻保存
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2019-04-23 , DOI: 10.1021/jacs.9b00992
Wei Zhu ₁ , Jimin Guo ₁ , Jacob Ongudi Agola ₁ , Jonas G Croissant ₁ , Zihao Wang ₁ , Jin Shang ₂ , Eric Coker ₃ , Benyamin Motevalli ₄ , Andreas Zimpel ₅ , Stefan Wuttke _{5,

6} , C Jeffrey Brinker ₁

Affiliation

模拟抗冻蛋白的混合纳米材料的开发可以调节/抑制用于细胞/组织冷冻保存的冰晶的生长，这引起了越来越多的兴趣。在此，我们描述了首次利用具有明确定义的表面化学性质的锆 (Zr) 基金属有机骨架 (MOF) 纳米粒子 (NPs) 来冷冻保存红细胞 (RBC)，而无需任何（有毒）有机物。溶剂。这种冷冻保护方法的显着特点包括卓越的水稳定性、低溶血活性以及 MOF NPs 表面有机连接体的长周期排列，这提供了氢供体的精确间距，以识别和匹配冰晶面。五种具有不同孔径、表面化学和骨架拓扑结构的 Zr 基 MOF NPs，用于红细胞的冷冻保护。“splat”试验证实，MOF NPs 不仅表现出冰重结晶抑制活性，而且还充当加速冰晶融化的“催化剂”。人类红细胞冷冻保存试验显示红细胞回收率高达 40%，高于通过常用羟乙基淀粉聚合物获得的回收率。这种冷冻保存方法将激发 MOF 衍生纳米结构的设计和利用，以有效地冷冻保存各种细胞类型和组织样本。人类红细胞冷冻保存试验显示红细胞回收率高达 40%，高于通过常用羟乙基淀粉聚合物获得的回收率。这种冷冻保存方法将激发 MOF 衍生纳米结构的设计和利用，以有效地冷冻保存各种细胞类型和组织样本。人类红细胞冷冻保存试验显示红细胞回收率高达 40%，高于通过常用羟乙基淀粉聚合物获得的回收率。这种冷冻保存方法将激发 MOF 衍生纳米结构的设计和利用，以有效地冷冻保存各种细胞类型和组织样本。

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Metal-Organic Framework Nanoparticle-Assisted Cryopreservation of Red Blood Cells

The development of hybrid nanomaterials mimicking antifreeze proteins that can modulate/inhibit the growth of ice crystals for cell/tissue cryopreservation has attracted increasing interests. Herein, we describe the first utilization of zirconium (Zr)-based metal-organic framework (MOF) nanoparticles (NPs) with well-defined surface chemistries for the cryopreservation of red blood cells (RBCs) without the need of any (toxic) organic solvents. Distinguishing features of this cryoprotective approach include the exceptional water stability, low hemolytic activity, and the long periodic arrangement of organic linkers on the surface of MOF NPs, which provide a precise spacing of hydrogen donors to recognize and match the ice crystal planes. Five kinds of Zr-based MOF NPs, with different pore size, surface chemistry, and framework topologies, were used for the cryoprotection of RBCs. A "splat" assay confirmed that MOF NPs not only exhibited ice recrystallization inhibition activities but also acted as a "catalyst" to accelerate the melting of ice crystals. The human RBC cryopreservation tests displayed RBC recoveries of up to ∼40%, which is higher than that obtained via commonly used hydroxyethyl starch polymers. This cryopreservation approach will inspire the design and utilization of MOF-derived nanoarchitectures for the effective cryopreservation of various cell types as well as tissue samples.

更新日期：2019-04-23

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