蓝，绿和红基分子印迹荧光传感器用于牛血红蛋白的多路复用和视觉检测的三元发射。,Analytical Chemistry

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蓝，绿和红基分子印迹荧光传感器用于牛血红蛋白的多路复用和视觉检测的三元发射。
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-04-23 00:00:00 , DOI: 10.1021/acs.analchem.9b00082
Qian Yang _{1,

2} , Jinhua Li ₁ , Xiaoyan Wang _{1,

3} , Hua Xiong ₂ , Lingxin Chen _{1,

4,

5}

Affiliation

通过以适当比例混合蓝/绿/红发射牛血红蛋白（BHb）印迹聚合物（b-MIP，g-MIP和r-MIP），提出了一种新型的三元发射荧光传感器BHb的多重视觉检测。这三个MIP分别嵌入有蓝色发光的7-羟基香豆素，绿色发光的CdTe量子点（QD）和红色发光的CdTe / ZnS量子点。在8分钟内与BHb相互作用后，CdTe和CdTe / ZnS QD的荧光同时关闭，而7-羟基香豆素打开了荧光强度。随即，三元发射的比例荧光强度在0.025–3μMBHb范围内线性变化，伴随着从黄绿色到黄色到鲑鱼到李子到紫色再到紫色到蓝色的大量荧光颜色演变。与双发射或单发射传感器相比，三发射荧光MIPs传感器提供了覆盖绿-红-蓝窗口的更宽的颜色变化，从而可以肉眼准确地确定BHb，并且检测限低至7.8 nM和更高的压印因子15.2。此外，在测定牛尿样品中加标的BHb时，其令人满意的99.25–111.7％的回收率，以及光学稳定性和印迹后构造的便利性，表明开发的三色发射荧光MIPs传感器为快速，快速，快速，准确的检测提供了理想的选择。灵敏，直观地测定复杂样品中的蛋白质。三元荧光MIPs传感器提供了覆盖绿-红-蓝窗口的较宽的颜色变化，可通过肉眼准确测定BHb，检测限较低，低至7.8 nM，印迹因子较高，为15.2。此外，在测定牛尿样品中加标的BHb时，其令人满意的99.25–111.7％的回收率，以及光学稳定性和印迹后构造的便利性，表明开发的三色发射荧光MIPs传感器为快速，快速，快速，准确的检测提供了理想的选择。灵敏，直观地测定复杂样品中的蛋白质。三元荧光MIPs传感器提供了覆盖绿-红-蓝窗口的较宽的颜色变化，可通过肉眼准确测定BHb，检测限较低，低至7.8 nM，印迹因子较高，为15.2。此外，在测定牛尿样品中加标的BHb时，其令人满意的99.25–111.7％的回收率，以及光学稳定性和印迹后构造的便利性，表明开发的三色发射荧光MIPs传感器为快速，快速，快速，准确的检测提供了理想的选择。灵敏，直观地测定复杂样品中的蛋白质。

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Ternary Emission of a Blue-, Green-, and Red-Based Molecular Imprinting Fluorescence Sensor for the Multiplexed and Visual Detection of Bovine Hemoglobin.

A novel ternary-emission fluorescence sensor was proposed by post-imprinting mixing blue-/green-/red-emission bovine hemoglobin (BHb) imprinted polymers (b-MIPs, g-MIPs, and r-MIPs) at a proper ratio and realized the multiplexed and visual detection of BHb. The three MIPs were individually embedded with blue-emission 7-hydroxycoumarin, green-emission CdTe quantum dots (QDs), and red-emission CdTe/ZnS QDs. Upon interaction with BHb within 8 min, the fluorescence of CdTe and CdTe/ZnS QDs were simultaneously turned off, whereas the 7-hydroxycoumarin turned on the fluorescence intensity. Thereupon, the ratiometric fluorescence intensity of the ternary emission linearly varied within 0.025–3 μM BHb, accompanying the profuse fluorescence color evolution from yellowish green to yellow to salmon to plum to purple to finally blue. In comparison with the dual- or single-emission sensor, the ternary-emission fluorescence MIPs sensor provided a wider color variation covering the green–red–blue window for accurate naked-eye determination of BHb, as well as a lower detection limit down to 7.8 nM and a higher imprinting factor of 15.2. Moreover, the satisfactory recoveries of 99.25–111.7% in determining the spiked BHb in bovine urine samples, as well as the optical stability and post-imprinting construction convenience, indicated that the developed tricolor-emission fluorescence MIPs sensor provided an ideal alternative for rapid, sensitive, and visual determination of proteins in complicated samples.

更新日期：2019-04-23

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