Core Catalysis of the Reductive Glycine Pathway Demonstrated in Yeast,ACS Synthetic Biology

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Core Catalysis of the Reductive Glycine Pathway Demonstrated in Yeast
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2019-04-19 00:00:00 , DOI: 10.1021/acssynbio.8b00464
Jorge Gonzalez de la Cruz ₁ , Fabian Machens ₂ , Katrin Messerschmidt ₃ , Arren Bar-Even ₁

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One-carbon (C1) compounds are attractive microbial feedstocks as they can be efficiently produced from widely available resources. Formate, in particular, represents a promising growth substrate, as it can be generated from electrochemical reduction of CO₂ and fed to microorganisms in a soluble form. We previously identified the synthetic reductive glycine pathway as the most efficient route for aerobic growth on formate. We further demonstrated pathway activity in Escherichia coli after expression of both native and foreign genes. Here, we explore whether the reductive glycine pathway could be established in a model microorganism using only native enzymes. We used the yeast Saccharomyces cerevisiae as host and show that overexpression of only endogenous enzymes enables glycine biosynthesis from formate and CO₂ in a strain that is otherwise auxotrophic for glycine. We find the pathway to be highly active in this host, where 0.125 mM formate is sufficient to support growth. Notably, the formate-dependent growth rate of the engineered S. cerevisiae strain remained roughly constant over a very wide range of formate concentrations, 1–500 mM, indicating both high affinity for formate use and high tolerance toward elevated concentration of this C1 feedstock. Our results, as well the availability of endogenous NAD-dependent formate dehydrogenase, indicate that yeast might be an especially suitable host for engineering growth on formate.

中文翻译：

酵母中还原型甘氨酸途径的核心催化

一碳（C1）化合物是有吸引力的微生物原料，因为它们可以从广泛可用的资源中高效生产。甲e尤其是一种有前途的生长底物，因为它可以通过电化学还原CO ₂生成并以可溶形式供入微生物。我们之前将合成的还原性甘氨酸途径确定为甲酸有氧生长的最有效途径。表达天然和外源基因后，我们进一步证明了大肠杆菌中的途径活性。在这里，我们探索是否可以仅使用天然酶在模型微生物中建立还原性甘氨酸途径。我们使用了酿酒酵母作为宿主，表明仅内源性酶的过表达就能使甘氨酸营养缺陷的菌株中的甲酸盐和CO ₂合成甘氨酸。我们发现该宿主中的途径高度活跃，其中0.125 mM的甲酸足以支持生长。值得注意的是，工程改造的酿酒酵母菌株的甲酸盐依赖性生长速率在很宽的甲酸盐浓度范围（1-500 mM）中保持大致恒定，这表明对甲酸盐的使用具有很高的亲和力，并且对这种C1原料的浓度具有较高的耐受性。我们的结果以及内源性NAD依赖的甲酸盐脱氢酶的可用性表明，酵母可能是特别适合在甲酸盐上进行工程生长的宿主。

更新日期：2019-04-19

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