Radopholus similis is an important migratory endoparasitic nematode, severely harms banana, citrus and many other commercial crops. Little is known about the molecular mechanism of infection and pathogenesis of R. similis. In this study, 64761 unigenes were generated from eggs, juveniles, females and males of R. similis. 11443 unigenes showed significant expression difference among these four life stages. Genes involved in host parasitism, anti-host defense and other biological processes were predicted. There were 86 and 102 putative genes coding for cell wall degrading enzymes and antioxidase respectively. The amount and type of putative parasitic-related genes reported in sedentary endoparasitic plant nematodes are variable from those of migratory parasitic nematodes on plant aerial portion. There were no sequences annotated to effectors in R. similis, involved in feeding site formation of sedentary endoparasites nematodes. This transcriptome data provides a new insight into the parasitic and pathogenic molecular mechanisms of the migratory endoparasitic nematodes. It also provides a broad idea for further research on R. similis.

Radopholus similis是一种重要的迁徙性内寄生线虫，严重危害香蕉，柑橘和许多其他商业作物。关于相似菌感染和发病机制的分子知之甚少。在这项研究中，从拟南芥的卵，幼虫，雌性和雄性中产生了64761个单基因。11443个单基因在这四个生命阶段之间表现出显着的表达差异。预测了与宿主寄生，抗宿主防御和其他生物学过程有关的基因。推定的基因分别有86个和102个编码细胞壁降解酶和抗氧化酶。在久坐的内生植物线虫中报道的推定的寄生虫相关基因的数量和类型与植物空中部分上的迁徙性寄生线虫的数量和类型不同。在拟南芥中没有注释效应子的序列，该序列参与久坐的内寄生线虫的进食位点形成。该转录组数据提供了对迁移性内寄生线虫的寄生和致病分子机制的新见解。它还为进一步的研究提供了广阔的思路。西米利斯河（R. similis）。