Production of l-Ribulose Using an Encapsulated l-Arabinose Isomerase in Yeast Spores,Journal of Agricultural and Food Chemistry

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Production of l-Ribulose Using an Encapsulated l-Arabinose Isomerase in Yeast Spores
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-04-16 00:00:00 , DOI: 10.1021/acs.jafc.9b00640
Xiaoxiao Liu ₁ , Zijie Li ₁ , Zhou Chen ₁ , Ning Wang ₁ , Yahui Gao ₂ , Hideki Nakanishi ₁ , Xiao-Dong Gao ₁

Affiliation

The rare sugar l-ribulose is produced from the abundant sugar l-arabinose by enzymatic conversion. An l-arabinose isomerase (AI) from Geobacillus thermodenitrificans was efficiently expressed and encapsulated in Saccharomyces cerevisiae spores. Deletion of the yeast OSW2 gene, which causes a mild defect in the integrity of the spore wall, substantially improved the activity of encapsulated AI, without damaging its superior enzymatic properties of thermostability, pH tolerance,and resistance toward SDS and proteinase treatments. In a 10 mL reaction, 100 mg of dry AI encapsulated in spores produced 250 mg of l-ribulose from 1 g of l-arabinose, indicating a 25% conversion rate. Notably, the product of l-ribulose was directly purified from the reaction solution with an approximately 91% recovery using a Ca²⁺ ion exchange column. Our results describe not only a facile approach for the production of l-ribulose but also a useful strategy for the enzymatic conversion of rare sugars in “Izumoring”.

中文翻译：

使用封装的酵母孢子中的l-阿拉伯糖异构酶生产l-核糖

稀有的糖1-核糖通过酶促转化由丰富的糖1-阿拉伯糖产生。来自热地芽孢杆菌的1-阿拉伯糖异构酶（AI）被有效地表达并包囊在酿酒酵母孢子中。酵母OSW2基因的缺失会在孢子壁的完整性中造成轻微缺陷，从而大大改善了被包封的AI的活性，而不会损害其优异的酶稳定性，热稳定性，pH耐受性以及对SDS和蛋白酶处理的抵抗力。在10 mL反应中，封装在孢子中的100 mg干AI从1 g的l中产生了250 mg的l-核糖。-阿拉伯糖，表示25％的转化率。值得注意的是，使用Ca ²⁺离子交换柱从反应溶液中直接纯化1-核糖产物，回收率约为91％。我们的结果不仅描述了生产1-核糖的简便方法，而且描述了“出雾”中稀有糖的酶促转化的有用策略。

更新日期：2019-04-16

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